This Article Full Text Full Text (PDF) All Versions of this Article: jhc.2009.954263v1 57/12/1183    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Yanchenko, N. Articles by Hattori, T. PubMed PubMed Citation Articles by Yanchenko, N. Articles by Hattori, T. Social Bookmarking                         What's this?

Application of a Novel Method of Double APAAP Staining With Subsequent Quantitative Image Analysis to the Examination of Integrin Expression in Undifferentiated-type Gastric Carcinomas

Natalia Yanchenko, Hiroyuki Sugihara and Takanori Hattori

Department of Pathology, Shiga University of Medical Science, Otsu, Shiga, Japan

Correspondence to: Natalia Yanchenko, Department of Pathology, Shiga University of Medical Science, Seta-Tsukinowa cho, Otsu-shi, 520-2192, Japan. E-mail: natalia.yanchenko{at}gmail.com

In undifferentiated-type gastric carcinoma (UGC), recognition of cancer cells is not easy, which has hampered its precise phenotypic analysis. To examine alterations of the integrin phenotype during the progression of UGC, we used double alkaline phosphatase anti-alkaline phosphatase staining and computer-aided image analyses for the expression of 1, 2, 3, 5, 6, V, β1, and β4 integrin subunits and Vβ3, Vβ5, and Vβ6 integrins in cytokeratin-positive cells in the mucosal, the submucosal, and the deeper parts of 10 early and 17 advanced UGCs, their non-neoplastic counterparts, and 9 lymph node (LN) metastases. We revealed declining expression of epithelial integrin subunits (2, 3, 6, β4) and increasing expression of mesenchymal integrin subunits (1, 5) as the tumor invaded deeper, reflecting gradual epithelial-to-mesenchymal transition of the integrin phenotype during tumor invasion. Enhanced expression of the V integrin subunit and Vβ3 and Vβ5 integrins correlated with tumor invasion, and that of Vβ6 integrins with LN metastasis. Our results have demonstrated that the method we introduced is suitable for analysis of dynamic alterations of the integrin repertoire in UGC progression. (J Histochem Cytochem 57:1183–1193, 2009)

Key Words: integrin • gastric carcinoma • EMT • double staining • image analysis

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2009