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Protein Tyrosine Phosphatase Interacting Protein 51 (PTPIP51) mRNA Expression and Localization and Its In Vitro Interacting Partner Protein Tyrosine Phosphatase 1B (PTP1B) in Human Placenta of the First, Second, and Third Trimester

Albrecht Stenzinger¹, David Märker¹, Philipp Koch, Jens Hoffmann, Nelli Baal, Klaus Steger and Monika Wimmer

Institute of Anatomy and Cell Biology (AS,DM,PK,JH,MW), Department of Obstetrics and Gynecology (NB), and Department of Urology and Pediatric Urology (KS), Justus-Liebig-University, Giessen, Germany

Correspondence to: Monika Wimmer, Institute of Anatomy and Cell Biology, Justus-Liebig-University, 35385 Giessen, Germany. E-mail: Monika.Wimmer{at}anatomie.med.uni-giessen.de

The cellular localization of protein tyrosine phosphatase 51 (PTPIP51) and its in vitro interacting partner protein tyrosine phosphatase 1B (PTP1B) was studied in human placentae of different gestational stages. The expression of PTPIP51 protein and mRNA was observed in the syncytiotrophoblast and cytotrophoblast layer of placentae from the first, second, and third trimesters. In contrast, PTP1B expression was restricted to the syncytiotrophoblast during all gestational stages. Cells of the cytotrophoblasts and parts of the syncytiotrophoblasts expressing high amounts of PTPIP51 were found to execute apoptosis as shown by TdT-mediated dUTP-biotin nick end labeling assay, cytokeratin 18f, and caspase 3 expression. PTPIP51 could also be traced in the endothelium and smooth muscle cells of placental arterial and venous vessels, identified by double immunostainings with antibodies directed against van Willebrand factor and -smooth muscle actin. Some of these cells showing a high PTPIP51 reactivity were Ki67 positive, indicating proliferation. Additionally, a small population of placental CD14-positive macrophages and mesenchymal cells within the villous stroma were detected as PTPIP51 positive. Our data suggest that both proteins, PTPIP51 and PTP1B, play a role in differentiation and apoptosis of the cytotrophoblast and syncytiotrophoblast, respectively. Moreover, PTPIP51 may also serve as a cellular signaling partner in angiogenesis and vascular remodeling. (J Histochem Cytochem 57:143–153, 2009)

Key Words: angiogenesis • apoptosis • differentiation • human • placenta • proliferation • protein tyrosine phosphatase 1B • protein tyrosine phosphatase interacting protein 51

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