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Expression of Muc19/Smgc Gene Products During Murine Sublingual Gland Development: Cytodifferentiation and Maturation of Salivary Mucous Cells

Biswadip Das, Melanie N. Cash, Arthur R. Hand, Armin Shivazad and David J. Culp

Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida (BD,MNC,AS,DJC), and Departments of Craniofacial Sciences and Cell Biology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut (ARH)

Correspondence to: Dr David J. Culp, Department of Oral Biology, University of Florida College of Dentistry, 1600 SW Archer Rd., PO Box 100424, Gainesville, FL 32610-3003. E-mail: dculp{at}dental.ufl.edu

Muc19/Smgc expresses two splice variants, Smgc (submandibular gland protein C) and Muc19 (mucin 19), the latter a major exocrine product of differentiated murine sublingual mucous cells. Transcripts for Smgc were detected recently in neonatal sublingual glands, suggesting that SMGC proteins are expressed during initial salivary mucous cell cytodifferentiation. We therefore compared developmental expression of transcripts and translation products of Smgc and Muc19 in sublingual glands. We find abundant expression of SMGC within the initial terminal bulbs, with a subsequent decrease as Muc19 expression increases. During postnatal gland expansion, SMGC is found in presumptive newly formed acinar cells and then persists in putative acinar stem cells. Mucin levels increase 7-fold during the first 3 weeks of life, with little change in transcript levels, whereas between postnatal days 21 and 28, there is a 3-fold increase in Muc19 mRNA and heteronuclear RNA. Our collective results demonstrate the direct transition from SMGC to Muc19 expression during early mucous cell cytodifferentiation and further indicate developmentally regulated changes in Muc19/Smgc transcription, alternative splicing, and translation. These changes in Muc19/Smgc gene expression delineate multiple stages of salivary mucous cell cytodifferentiation and subsequent maturation during embryonic gland development through the first 4 weeks of postnatal life. (J Histochem Cytochem 57:383–396, 2009)

Key Words: mucin • exocrine • alternative splicing • salivary glands

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				B. Das, M. N. Cash, A. R. Hand, A. Shivazad, S. S. Grieshaber, B. Robinson, and D. J. Culp Tissue Distibution of Murine Muc19/Smgc Gene Products J. Histochem. Cytochem., February 1, 2010; 58(2): 141 - 156. [Abstract] [Full Text] [PDF]

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