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Originally published as JHC exPRESS on January 5, 2009.
doi:10.1369/jhc.2008.951814
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Journal of Histochemistry and Cytochemistry
Volume 57 (5): 405-423, 2009
Copyright ©The Histochemical Society, Inc.

Region-specific Differentiation Potential of Adult Rat Spinal Cord Neural Stem/Precursors and Their Plasticity in Response to In Vitro Manipulation

Iris Kulbatski and Charles H. Tator

Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada, and Toronto Western Research Institute, Krembil Neuroscience Center, University Health Network, Toronto, Ontario, Canada

Correspondence to: Iris Kulbatski, 399 Bathurst Street, McLaughlin Pavilion 12-423, Toronto, Ontario M5T 2S8, Canada. E-mail: iris.kulbatski{at}sympatico.ca

This study characterized the differentiation of neural stem/precursor cells (NSPCs) isolated from different levels of the spinal cord (cervical vs lumbar cord) and different regions along the neuraxis (brain vs cervical spinal cord) of adult male Wistar enhanced green fluorescent protein rats. The differentiation of cervical spinal cord NSPCs was further examined after variation of time in culture, addition of growth factors, and changes in cell matrix and serum concentration. Brain NSPCs did not differ from cervical cord NSPCs in the percentages of neurons, astrocytes, or oligodendrocytes but produced 26.9% less radial glia. Lumbar cord NSPCs produced 30.8% fewer radial glia and 6.9% more neurons compared with cervical cord NSPCs. Spinal cord NSPC differentiation was amenable to manipulation by growth factors and changes in in vitro conditions. This is the first study to directly compare the effect of growth factors, culturing time, serum concentration, and cell matrix on rat spinal cord NSPCs isolated, propagated, and differentiated under identical conditions. (J Histochem Cytochem 57:405–423, 2009)

Key Words: neural precursors • region specificity • differentiation • spinal cord • myelination • growth factors • in vitro • co-culture • serum • cell matrix


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