This Article Full Text Full Text (PDF) All Versions of this Article: jhc.2009.952960v1 57/7/665    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Miranda, F. A. Articles by Soares, E. G. Search for Related Content PubMed PubMed Citation Articles by Miranda, F. A. Articles by Soares, E. G. Social Bookmarking                         What's this?

Galectin-3 Overexpression in Invasive Laryngeal Carcinoma, Assessed by Computer-assisted Analysis

Fabiana A. Miranda, Marcela K. Hassumi, Marcia C.M. Guimarães, Renata T. Simões, Tarsia G.A. Silva, Régia C.P. Lira, Ana M. Rocha, Celso T. Mendes, Jr., Eduardo A. Donadi, Christiane P. Soares and Edson G. Soares

Department of Pathology, Faculty of Medicine (FAM,MKH,MCMG,RCPL,AMR,EGS), Division of Clinical Immunology, Department of Medicine, Faculty of Medicine (RTS,EAD), and Department of Chemistry, Faculty of Philosophy, Science and Letters (CTM) of Ribeirão Preto, University of São Paulo, São Paulo, Brazil, and Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University, São Paulo, Brazil (TGAS,CPS)

Correspondence to: Fabiana Alves Miranda, Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil. E-mail: famiranda{at}usp.br

The larynx is the most common site of malignancy in the upper aerodigestive tract. In Brazil, malignant laryngeal lesions represent 2% of all cancers, with 3000 annual deaths. The association between human papillomavirus (HPV) and laryngeal cancer is still controversial. The aim of the present retrospective study was to determine the expression of galectin-3 immunoperoxidase in laryngeal carcinoma by examining paraffin-embedded larynx biopsies from 65 patients, 10 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastases, and 28 with metastases. Twenty-eight cervical lymph nodes from patients with metastatic lesions were also evaluated. Nested PCR was performed to detect and type HPV DNA. Galectin-3 expression was assessed by immunohistochemistry using a computer-assisted system. Among 65 patients, 55 (84.6%) were positive to beta-globin (internal control); 10 (15.4%) patients were beta-globin negative and were excluded from the HPV evaluation. Thus, 7 (12.7%) out of 55 patients were HPV positive and 48 (87.3%) out of 55 patients were HPV negative. High expression of galectin-3 was observed in invasive laryngeal tumors, suggesting that galectin-3 could be associated with the invasiveness and aggressiveness of laryngeal carcinomas. (J Histochem Cytochem 57:665–673, 2009)

Key Words: galectin-3 • human papillomavirus • laryngeal carcinoma • immunohistochemistry • PCR

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2009