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Originally published as JHC exPRESS on April 13, 2009.
doi:10.1369/jhc.2009.953646
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Journal of Histochemistry and Cytochemistry
Volume 57 (8): 741-751, 2009
Copyright ©The Histochemical Society, Inc.

Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes

Yan-Chao Li, Wan-Zhu Bai, Kazuhisa Sakai and Tsutomu Hashikawa

Neural Architecture, Advanced Technology Development Group, RIKEN Brain Science Institute, Saitama, Japan

Correspondence to: Yan-Chao Li, Neural Architecture, Advanced Technology Development Group, RIKEN Brain Science Institute, Hirosawa 2-1, Wako, Saitama 351-0198, Japan. E-mail: ldlyc{at}brain.riken.jp or ldlyc{at}yahoo.com

The organization of F-actin in the ventricular system has been reported to display pronounced regional differences with respect to shape, size, and development. However, the real roles played by F-actin in these cells cannot be understood unless the precise localization of F-actin is defined. In the present study, we used double-fluorescence labeling to further examine the localization of F-actin in the ependymocytes and its spatial relation to the other two cytoskeletal components, microtubules and intermediate filaments. Then we converted fluorescence signals for F-actin to peroxidase/DAB reaction products by use of a phalloidin-based FITC-anti-FITC system. This detection technique provided an overview of the distribution of F-actin in the ependymocytes at the ultrastructural level, and has been proven to be helpful in correlating light and electron microscopic investigations. (J Histochem Cytochem 57:741–751, 2009)

Key Words: F-actin • ependyma • rat • confocal microscopy • electron microscopy


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