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HISTOCHEMICAL DEMONSTRATION OF beta-D-GALACTOSIDASE IN THE RAT

ALEXANDER M. RUTENBURG 1, SELMA H. RUTENBURG 1, BENITO MONIS 1, RUTH TEAGUE 1, and ARNOLD M. SELIGMAN 1

1 From the Departments of Surgery, Beth Israel Hospital and Harvard Medical School, Boston; Sinai Hospital of Baltimore and the Johns Hopkins University School of Medicine, Baltimore

A histochemical technic for demonstration of beta-d-galactosidase in formalin fixed sections has been presented. Enzymatic activity was best demonstrated in frozen sections of the kidney, liver, gastrointestinal tract, testis, epididymis and vas deferens of the rat and to a lesser extent in salivary gland, pancreas, thyroid, trachea, lung, spleen and lymphoid tissue. Leucocytes stained intensely. Enzymatic activity was largely present in the cytoplasm of epithelial cells. Cell nuclei, myocardium, connective tissue and erythrocytes were inactive.

Submitted on September 6, 1957


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