Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by KUWABARA, T.
Right arrow Articles by COGAN, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by KUWABARA, T.
Right arrow Articles by COGAN, D. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

TETRAZOLIUM STUDIES ON THE RETINA: I. INTRODUCTION AND TECHNIQUE

TOICHIRO KUWABARA 1 and DAVID G. COGAN 1

1 Howe Laboratory of Ophthalmology, Harvard University Medical School, and Massachusetts Eye and Ear Infirmary, Boston 14, Massachusetts

The retina has physical dimensions that make it especially favorable for incubation studies in its unsectioned state.

With blue tetrazolium anti suitable substrates, dehydrogenase activity is demonstrated in various, but characteristic, forms and places. A red color that depends on enzymatic activity is found in the reticular layers, nerve fiber layer, and lipid portions of the retina. This appears to represent a solubilization of reduced and partially reduced formazan in the lipid portions of the retina. The blue precipitate is found in mitochondria-like granules within certain cells, notably in the ellipsoids of the rods and cones, glial cells, Müller's cells, and to some extent in ganglion cells. It is also found as amorphous and rod-like crystalline deposits in the inner layers of the retina and as a fine dust-like deposit in the nerve fiber layer.

Metabolic activity in the retina as reflected by tetrazolium precipitation persists for hours after death and is present about equally in peripheral and central regions of the retina. The reduction brought about by the utilization of specific substrates representing intermediates in the major metabolic pathways will be the subject of subsequent papers of this series.

Submitted on March 9, 1959


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
F. P. Ruggiero and J. B. Sheffield
The Use of Avidin as a Probe for the Distribution of Mitochondrial Carboxylases in Developing Chick Retina
J. Histochem. Cytochem., February 1, 1998; 46(2): 177 - 184.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1959