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A NITROSOPHENOL REACTION FOR TYROSINE AND RELATED COMPOUNDS IN TISSUE SECTIONS

G. G. GLENNER 1

1 Laboratory of Pathology and Histochemistry N.I.A.M.D., National Institutes of Health, Bethesda 14, Maryland

1. A method for the histochemical localization of protein-bound tyrosine and other ortho unsubstituted substituted phenols by the formation of a nitrosophenol-metal chelate is described.

2. The histochemical formation of a nitrosophenol confirms the postulated first step in the diazotization-coupling reaction for the histochemical demonstration of protein-bound tyrosine.

3. Though the described method is useful as a confirmatory technique for the localization of protein-bound tyrosine, its sensitivity is markedly less than that of the diazotization-coupling reaction.

Submitted on May 15, 1959


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