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"pH SIGNATURE" OF ADENOCARCINOMA OF THE COLON IN MAN

ALVAN G. FORAKER 1

1 Research Laboratory, Baptist Memorial Hospital, Jacksonville, Florida

Sections from ten cases of adenocarcinoma of the human colon were stained with methylene blue and with light green SF in a range of pH. Staining intensity of various cellular structures in normal and neoplastic mucosa was recorded on an arbitrary scale. This procedure is said to assist in characterization of proteins. Nuclear structures stained more intensely with methylene blue in alkaline ranges. Mitotic figures in carcinoma retained affinity for methylene blue in relatively acid ranges when other nuclear structures stained weakly. Cytoplasmic staining with light green increased in acid ranges. Only mucin showed some metachromasia. None of the findings sharply differentiated between normal and carcinoma cells.

Submitted on November 27, 1959


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