Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WOLF, M. K.
Right arrow Articles by ARONSON, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WOLF, M. K.
Right arrow Articles by ARONSON, S. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

GROWTH, FLUORESCENCE AND METACHROMASY OF CELLS CULTURED IN THE PRESENCE OF ACRIDINE ORANGE

MERRILL K. WOLF 1 and SAMUEL B. ARONSON 1

1 National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda 14, Maryland

Four types of animal cells were cultured in perfusion chambers in medium containing acridine orange and serially observed by phase and fluorescence microscopy, so that cellular injury from toxic and photodynamic effects of acridine orange could be correlated with the variable fluorescence images of the cells. Spontaneous movements of pigment granules in healthy chorioidal and ciliary melanocytes provided a valuable criterion of cellular health. Dye concentrations of 10 –6 gm/ml permitted healthy outgrowth of brilliantly fluorescent cells for at least 20 days, but higher concentrations were lethal. Uninjured living cells had orthochromatic (green) fluorescence. Reversibly injured cells acquired metachromatic (red-fluorescent) cytoplasmic granules which might be identical with mitochondria or with the neutral red granules of Jackson. With irreversible photodynamic injury, ribonucleic acid of nucleoli and cytoplasm stained metachromatically; deoxyribonucleic acid of nuclei alnsost always remained orthochromatic. As degeneration ensued, cells lost first their metachromasy, finally all their fluorescence. The variable fluorescence images of living, injured, killed, degenerated and fixed cells stained with acridine orange may be explained according to the theory of variable stacking of bound dye molecules.

Submitted on January 4, 1960


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
F. Nadrigny, D. Li, K. Kemnitz, N. Ropert, A. Koulakoff, S. Rudolph, M. Vitali, C. Giaume, F. Kirchhoff, and M. Oheim
Systematic Colocalization Errors between Acridine Orange and EGFP in Astrocyte Vesicular Organelles
Biophys. J., August 1, 2007; 93(3): 969 - 980.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
I. Tasaki, L. Carnay, R. Sandlin, and A. Watanabe
Fluorescence Changes during Conduction in Nerves Stained with Acridine Orange
Science, February 14, 1969; 163(3868): 683 - 685.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1961