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PREPARATION OF NAPHTHOL AS-TR PHOSPHATE FOR LOCALIZATION OF PHOSPHATASE

GRACE M. JEFFREE 1 and KENNETH B. TAYLOR 1

1 Pathology Research Laboratory, University of Bristol, 18 Guinea Street, Bristol 1, England

The use of PCl5 in dioxan for the conversion of Naphthol AS-TR to the acid phosphate, even without the addition of catalysts other than pyridine, gave a conversion considerably superior to that obtained using POCl3 in either tetra-hydrofuran or dioxan, with or without the additional catalysts suggested by Burstone. The product proved satisfactory for histochemical localization of alkaline phosphatase in bone, though it is suspected that a certain amount of polymerization had occurred in the process of phosphorylation.

Submitted on March 10, 1960


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