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AN AUTORADIOGRAPHIC STUDY OF THE DISTRIBUTION OF ZINC-65 IN RAT TISSUES

M. J. MILLAR 1, N. R. VINCENT 1, and C. A. MAWSON 1

1 Biology Branch, Biology and Health Physics Division, Atomic Energy of Canada Ltd., Chalk River, Ontario, Canada

A new method of preparing rats for 65Zn autoradiography has been described. By ensuring a low excretion rate of 65Zn, autoradiographs with good cellular detail were obtained. The method used for mounting stripping film prevented contact of water with the tissue except during floating-out of the sections.

Autoradiographs of the lateral lobes of the dorsolateral prostate, epididymis, testis, ileum, pancreas and coat hair follicles are illustrated. 65Zn was concentrated at the base and lumen surfaces of the acinar cells in the lateral lobes of the dorsolateral prostate, in the tail pieces of testicular spermatozoa, in epididymal spermatozoa, in the Paneth cells of the ileum, in the pancreatic islets of Langerhans and in the shaft of the hair follicles. 65Zn distribution in liver, submaxillary glands, esophagus, pituitary, adrenals and kidney is also described.

Submitted on April 1, 1960


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