Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MANCINI, R. E.
Right arrow Articles by FIORINI, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MANCINI, R. E.
Right arrow Articles by FIORINI, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A HISTOCHEMICAL AND RADIOAUTOGRAPHIC STUDY OF THE PARTICIPAPATION OF FIBROBLASTS IN THE PRODUCTION OF MUCOPOLYSACCHARIDES IN CONNECTIVE TISSUE

R. E. MANCINI 1, O. VILAR 1, E. STEIN 1, and H. FIORINI 1

1 Instituto de Anatomia General y Embriologia, Facultad de Ciencias Medicas, Universidad de Buenos Aires, Argentina

A histochemical and radioautographic study of the connective tissue of rat skin was carried out from birth to maturity. The following observations were made:

1) Early fibroblasts changed into typical adult connective tissue cells, fibrocytes, with gradual diminution of cytoplasmic nucleoproteins, periodic acid Schiff positive material, succinodehydrogenase, beta-glucuronidase and leucyl-aminopeptidase.

2) Lack of striking morphological or cytochemical changes of the mast cells in the different ages as compared with fibroblasts, but more intense enzymatic reactions with respect to succinodehydrogenase, beta-glucuronidase, leucyl-aminopeptidase, alkaline and acid phosphatase, lipase esterase and cytochrome oxydase were found.

3) Using adenine-C14 and methionine-S35, the localization of these substances was seen more in the fibroblasts and mast cells than in the intercellular ground substance; in the fibroblasts the uptake was higher in the embryonic and early postnatal connective tissues than in the adult.

4) The embryonic and early postnatal connective tissues to synthesize sulphated polysaccharides trapped more S35 than the adult. S35 was seen first in the fibroblasts and then in the intercellular ground substance. The same results were observed in tissue cultures of fibroblasts. Mast cells incorporated and stored more S35 than fibroblasts, but there was no appreciable difference in their capacity for this at various ages, as there was in fibroblasts.

Submitted on July 21, 1960


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1961