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Journal of Histochemistry and Cytochemistry, Vol. 47, 1645b-1645, December 1999, Copyright © 1999, The Histochemical Society, Inc.

PROCEEDINGS

12 Standardization of biological dyes and stains through use of pH-zone-refining countercurrent chromatography

Adrian Weisza and Yoichiro Itob
a Office of Cosmetics and Colors, U.S. Food and Drug Administration, Washington, DC 20204,
b Laboratory of Biophysical Chemistry, NHLBI, National Institutes of Health, Bethesda, MD 20892,

The presence of contaminants in dyes used for biological staining can cause variations in their staining properties. This problem has been documented as the cause of anomalous histochemical staining results. A prerequisite for the standardization of biological stains is the availability of pure dyes that do not vary in composition from batch to batch. pH-Zone-refining countercurrent chromatography (CCC) has been recently developed as a preparative-scale method for the purification of multigram quantities of closely-related dyes (including positional isomers). In this presentation, it is proposed that pH-zone-refining CCC would facilitate the attainment (qualitatively and quantitatively) of the pure dyes necessary for the standardization of biological stains.


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