PROCEEDINGS

32 Molecular Cloning and Expression of Glomerular Epithelial Protein 1 (GLEPP1) in Developing Mouse Kidney

GLEPP1, a membrane tyrosine phosphatase, is found exclusively on glomerular podocytes of adult kidneys and chiefly on foot processes of these cells. To investigate the expression patterns of GLEPP1, we cloned its full length cDNA from newborn mouse kidney. Computer sequence analysis showed that mouse GLEPP1 encoded a protein of ~135 kD, was ~91% identical to human and rabbit GLEPP1, and contained 9-11 additional amino acid residues in the ectodomain. Northern analysis showed a transcript of ~6 kb in E15 and newborn kidney that became ~3 fold more abundant in adult kidney. In situ hybridization showed that GLEPP1 mRNA was first detected in immature podocytes of comma- and S-shaped nephric structures. GLEPP1 protein was not seen by immunofluorescence or immunogold electron microscopy until the capillary loop stage however, when it was found on all membrane surfaces of developing podocytes. As these cells differentiated, GLEPP1 protein was detected most abundantly on foot processes, and no specific labeling was found elsewhere in the kidney. Although the ligand for GLEPP1 is not known, its expression pattern is consistent for a role in podocyte foot process development and maintenance.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wang, R. Articles by Abrahamson, D. R. Search for Related Content PubMed PubMed Citation Articles by Wang, R. Articles by Abrahamson, D. R. Social Bookmarking                      What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact