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doi:10.1369/jhc.4A6540.2005
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Volume 53 (10): 1203-1214, 2005
Copyright ©The Histochemical Society, Inc.
Ultrastructural Studies and Na+,K+-ATPase Immunolocalization in the Antennal Urinary Glands of the Lobster Homarus gammarus (Crustacea, Decapoda)
Equipe Adaptation Ecophysiologique et Ontogenèse, Université Montpellier II, Montpellier, France (SK,GC,MC-D), and Faculty of Marine Sciences, University of Tarbiat Modarres, Tehran, Iran (SK)
Correspondence to: Mireille Charmantier-Daures, Equipe Adaptation Ecophysiologique et Ontogenèse, UMR 5171 GPIA, Université Montpellier II, cc 092, 34095 Montpellier cedex 05, France. E-mail: charmantier.daures{at}univ-montp2.fr
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Summary |
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Unlike in crustacean freshwater species, the structure and ultrastructure of the excretory antennal gland is poorly documented in marine species. The general organization and ultrastructure of the cells and the localization of Na+,K+-ATPase were examined in the antennal gland of the adult lobster Homarus gammarus. Each gland is composed of a centrally located coelomosac surrounded ventrally by a labyrinth divided into two parts (I and II) and dorsally by a voluminous bladder. There is no differentiated nephridal tubule between them. The labyrinth and bladder cells have in common a number of ultrastructural cytological features, including basal membrane infoldings associated with mitochondria, apical microvilli, and cytoplasmic extrusions, and a cytoplasm packed with numerous vacuoles, vesicles, lysosome-like bodies, and swollen mitochondria. Each type of cell also presents distinctive characters. Na+,K+-ATPase was detected through immunofluorescence in the basal part of the cells of the labyrinth and in the bladder cells with an increasing immunostaining from labyrinth I to the bladder. No immunoreactivity was detected in the coelomosac. The cells of the labyrinth and of the bladder present morphological and enzymatic features of ionocytes. The antennal glands of the lobster thus possess active ion exchanges capabilities. (J Histochem Cytochem 53:1203–1214, 2005)
Key Words: excretory organ • osmoregulation • fine structure • immunolocalization • Na+,K+-ATPase
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Introduction |
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IN DECAPODS CRUSTACEANS, three organs, the gut, the antennal glands and the branchial chambers, are involved in excretory and ion-regulatory functions. The urinary system radically differs from the other two in that its input fluid is entirely internal (i.e., hemolymph), whereas the branchial chambers and, to a lesser extent, the gut can directly interact with both the internal and external media. The excretory system of decapods consists of paired antennal glands, which rest in the basal antennal segment of the cephalothorax. The hemolymph supply of the antennal glands originates mainly from the antennal and the sternal arteries. As with the vertebrate kidney, these organs participate in maintaining the volume of the extracellular fluid and regulating its concentration in ions, nutrients, and other solutes (Mantel and Farmer 1983
). In marine decapods, besides the control of hemolymph volume, the antennal glands are involved in hyporegulation of hemolymph magnesium and sulfate, excretion of organic compounds, and reabsorption of fluid, sugars, and amino acids from the primary urine filtrate (Riegel and Cook 1975; Mantel and Farmer 1983; Fuller et al. 1989; Péqueux 1995). In the lobster Homarus americanus, the antennal glands are involved in calcium uptake (Ahearn and Franco 1993), in the reabsorption and transport of glucose (Burger 1957; Behnke et al. 1998), and of at least one amino acid, L-proline (Behnke et al. 1990).
From an osmoregulatory point of view, the juveniles and adults of homarid lobsters are osmoconformers in seawater and are slight hyperregulators in dilute media (Dall 1970; Charmantier et al. 1984,2001). Osmoregulation in these animals is apparently achieved through hyperregulation of Na+ and, to a lesser extent, of Cl–. K+ is hyporegulated at high salinities, and Mg2+ is strongly hyporegulated at all salinities (Charmantier et al. 1984).
Because of the active implication of the antennal glands in osmoregulation of freshwater decapods through the production of dilute urine, their structure has been more intensively studied in these species than in closely related marine species. This is particularly true in the Astacidea, in which the structure of the antennal glands has been described in several species of crayfish (review in Khodabandeh et al. 2005a). These studies have shown that each antennal gland is composed of a single nephron-like unit including the coelomosac, the labyrinth, the nephridial tubule (nephridial canal), and the urinary bladder. Ultrafiltration occurs across the wall of the coelomosac, which presents typical podocytes. The filtrate then flows through the different parts of the efferent duct, where it is modified through absorption and secretion processes. The labyrinth, nephridial tubule, and even the bladder are involved in salt reabsorption, which results in the production of urine that is hyposmotic to the hemolymph of crayfish (Riegel 1966; Riegel and Cook 1975; Susanto and Charmantier 2000,2001; Mc Mahon 2002; Vogt 2002; Khodabandeh et al. 2005b).
Although several physiological studies have been conducted on the antennal glands of homarid lobsters, no structural information is available on them except for the pioneering observations of Waite (1899) and Peters (1935). The nephridial canal, with its salt-reabsorbing function in crayfish, does not appear as compulsory in marine species such as homarid lobsters (Burger 1957; Dall 1970).
The aim of the present study was to: (a) describe the structure and ultrastructure of the different parts of the antennal glands in the adult European lobster H. gammarus and (b) locate potentially ion-transporting cells and localize Na+,K+-ATPase. Investigations were conducted through light and electron microscopy and through immunofluorescence for the enzyme detection.
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Materials and Methods |
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Animals
Adult H. gammarus caught off the coast of Brittany and obtained from a shellfish retailer (Les Viviers de Roscoff, Roscoff, France) were maintained at the Montpellier laboratory in individual compartments containing aerated and recirculated (Eheim Systems) natural seawater (35.0 ± 1.3%, 20 ± 1C) and were fed mussels three times per week. The photoperiod was held constant at 12L:12D.
Histology
The antennal glands were surgically removed from cold-anesthetized lobsters. For light microscopy observations, sample fixations and other histological processes were performed as previously described (Khodabandeh et al. 2005a).
Electron Microscopy
For transmission electron microscopy, samples were fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer for 24 hr at room temperature, pH 7.4, adjusted to 1030 mosmol/kg by NaCl to avoid osmotic shock. They were then rinsed in sodium cacodylate buffer, and postfixed for 1 hr in a mixture (v/v) of 2% osmium tetroxide and 0.45 M sodium cacodylate buffer at room temperature. Other processes of the ultrastructural observations techniques have been described in Khodabandeh et al. (2005a).
For scanning electron microscopy, samples were placed in cold 4% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4, containing 5% sucrose. After an initial 1 hr fixation, followed by rinsing in 0.1 M phosphate buffer containing 5% sucrose, the samples were postfixed for 1 hr in 2% osmium tetroxide in 0.1 M phosphate buffer, pH 7.4, containing 5% sucrose.
Immunocytochemistry
Immunolocalization of the Na+,K+-ATPase was performed through immunofluorescence light microscopy using a mouse monoclonal antibody IgG
5 raised against the -subunit of the chicken Na+,K+-ATPase (Takeyasu et al. 1988) obtained from the Development Studies Hybridoma Bank, developed under the auspices of the NICHD, and maintained by the University of Iowa. In crustaceans, this antiserum has previously been used to quantify the expression of the subunit after a dilute transfer in Carcinus maenas (Lucu and Flik 1999) and to localize the Na+,K+-ATPase in the branchial cavity of Homarus gammarus (Lignot and Charmantier 1999; Lignot et al. 2001) and in the gills (Lignot et al. 2005) and antennal glands (Khodabandeh et al. 2005b) of Astacus leptodactylus.
After 24 hr in Bouin's fixator and embedment in paraplast, sections of 3–5 µm were cut on a Leitz Wetzlar microtome and collected on poly-L-lysine–coated slides. The immunocytochemistry procedure has been previously described (Lignot et al. 2001,2005; Khodabandeh et al. 2005b). Posterior gills of the crab Pachygrapsus marmoratus were tested each time as positive and specific control.
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General Organization
In adult H. gammarus, the antennal gland as seen from above is triangular in shape and flattened (Figure 1A). The bottom of the hilus is close to the center of the gland (Figure 1A). The antennal gland presents three lobes, two external and internal anterior lobes and one posterior main lobe (Figure 1A). It lies on the ventral floor of the cephalothorax, but part of the anterior lobes extends into the basis of the antenna. Each gland is composed of three regions: the coelomosac, the labyrinth, and the bladder linked by a short duct to the urinary pore. The coelomosac is located on the dorsal part of the labyrinth and it possesses a central cavity (Figures 1B and 1C). The dorsal face of the coelomosac is in close contact with the ventral wall of the overlying bladder (Figure 1C). The coelomosac region displays regularly organized cavities limited by single layer of cells (Figure 1E and Figure 2A). The cells form many complete and incomplete septa in the wall of the coelomosac, which are more abundant on the ventral than on the dorsal wall (Figures 1D and 1E; Figure 2A). Each septum is constituted by a folded epithelium embracing a sheet of connective tissue between its two layers (Figure 1E). The connective tissue contains hemolymph vessels. The opening of the coelomosac to the underlying labyrinth is located in the main lobe of the gland immediately behind the hilus (Figure 1B). This connection is the only location where the cells of the coelomosac and of the labyrinth come into contact.
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The labyrinth constitutes the greater bulk of the gland (Figures 1B and 1C). Its ventral part is covered by a connective tissue (Figures 1C and 1D). This tissue is highly vascular, mainly formed by the walls of hemolymph vessels and hemolymph spaces (not shown). The labyrinth (Figures 1B–1D) consists of a very complicated spongy-like tissue. The space between the epithelial lining is occupied by a connective tissue (Figures 1C and 1D). The dorsal face of the labyrinth is covered by the coelomosac, except in the border areas (Figure 1C). In this region, two subregions have been observed, labyrinth I and II. Labyrinth I immediately follows the coelomosac and forms the main part of the labyrinth. Labyrinth II is present only in the lateral border of the labyrinth where it connects with the bladder (Figure 1C). The lumen of this subregion is considerably reduced compared with the lumen of labyrinth I (not shown).
The bladder (Figure 1C) is a large reservoir, firmly attached to the dorsal face of the gland. Between the basal membrane of the epithelium of the bladder (ventral wall) and the coelomosac lies a rich plexus of vessels. The bladder (Figures 1F and 1G) is lined by a single layer of epithelial cells covered by conjunctive tissue, and a system of hemolymph vessels is present in between. The thickness of the conjunctive layer is more variable than that of the epithelial layer (Figure 1G). It is composed of flattened cells with a centrally located nucleus, organized into one to several cellular layers.
Ultrastructure
Ultrastructurally, the coelomosac cells (podocytes) present a variable shape. They possess distinctive pedicels or foot processes (Figure 2B) that extend to the basal plasma membrane separating the coelomosac cell from the underlying hemolymph space. The apical portions of all cells are devoid of microvilli and expand into voluminous globules, which are often two times larger in diameter than the basal portion (Figures 1A and 1B). These globular vesicles contain spherical structures that become detached into the lumen (Figure 2C). Consequently, the lumen of the coelomosac is more or less filled with different and various-sized globules that contain residual bodies (Figures 2A and 2C). The cells are separated by large intercellular space, but they are bound together by desmosome-like intercellular attachments. The main parts of the cells contain large intracellular dense bodies, mitochondria, vesicles, and vacuoles (Figure 2B). Typical Golgi complex systems are generally observed, consisting of the usual stacks of concentric or flattened cisternae (Figure 2B). The nucleus, round or oval with a voluminous nucleolus, is usually situated in the apical half part of the cells (Figure 2B).
In all parts of the labyrinth and of the bladder, the cells present several common features, including apical microvilli, apical cytoplasmic extrusions (Figure 2D; Figure 3A; Figures 4A and 4B; Figures 5A and 5B), and basal membrane infoldings associated with mitochondria (Figure 2A; Figure 3C; Figure 4C; Figure 5B). The arrangement of apical microvilli is usually disturbed by the formation of cytoplasmic extrusions that become detached and pass into the lumen (Figure 2D; Figure 3A; Figure 4A; Figure 5A). The cytoplasm contains lysosome-like bodies, abundant clear vacuoles of different sizes, dense bodies, mitochondria, and glycogen granules. Beside the typical small-sized mitochondria, other clear and voluminous mitochondria with small tubular cristae are observable (Figure 4D and Figure 5E). In the labyrinth I, two cell types are observable, columnar cells and cuboidal cells. (a) Columnar cells (Figure 2D) (average height 22 µm) possess an apical nucleus, dilated intercellular spaces, irregular apical microvilli, and irregular basal membrane infoldings associated with mitochondria. The large globular vesicles and cytoplasmic extrusions in these cells contain numerous small vesicles, vacuoles, and, frequently, dense spherical residual bodies. As in the coelomosac region, spherical residual bodies are often observed in the globular vesicles and in the lumen (not shown). (b) In cuboidal cells (EFigures 3A–5E) (height 20 µm), the nucleus is centrally located; the main features of these cells include the presence of regular basal infoldings associated with round mitochondria, globules of glycogen, and lysosome-like bodies (Figure 3C). They display many apical swollen mitochondria, tight junctions, and zonula adherens (Figure 3E). Their apical large globular vesicles contain many vacuoles and swollen mitochondria, and no spherical structure is observable (Figure 3B). Vacuoles, lysosome-like bodies, glycogen globules, and mitochondria are consistently observable in close contact (Figure 3D). The labyrinth II cells (Figures 4A–4E) are columnar-shaped (average height 27 µm) and their cytoplasm is usually clear. On their basal side, short basal infoldings are associated with mitochondria, vesicles, and numerous and large vacuoles containing fine particles (Figure 4C). Their apical portion and cytoplasmic extrusions contain clear vacuoles, small vesicles, mitochondria, and lysosome-like bodies (Figures 4A and 4D). Besides these cytoplasmic extrusions, well-developed vacuoles and large, globular vesicles containing fine particles of labyrinth II cells bulge into the lumen and are free from the cell body (Figures 4B and 4E).
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The bladder cells (Figures 5A–5E) present different sizes according to their location. The ventral wall of the bladder is lined by a thin epithelium (average height 15 µm), whereas the lateral and dorsal walls are limited by a thick epithelium (average height 45 µm). The bladder cells are columnar with an apical nucleus and irregular apical microvilli. They are filled with numerous and clear vacuoles of different sizes containing fine particles, frequently surrounded by an electron-dense cisterna, or associated with dense bodies and lysosome-like bodies (Figures 5B and 5E). They possess apical cytoplasmic extrusions into the lumen that contain clear vacuoles; there is no apical globular vesicle (Figures 5C and 5D). Small endocytic vesicles are frequently present in the apical region (Figures 5C and 5D). As in the labyrinth cells, clear and voluminous mitochondria with small tubular cristae are observable in the apical extrusions (Figures 5A and 5D). The basal side of the cells is dense and presents deep membrane infoldings associated with mitochondria, sparse vacuoles, and small, clear vesicles (Figure 5B). The bladder cells possess a thick basal lamina (Figure 5B).
Immunocytochemistry
Immunofluorescence was used for the localization of the Na+,K+-ATPase, and the fixation and staining process leaves a good antigenicity. Positive control crab sections were constantly brightly immunostained (not shown). Negative control sections showed no specific binding within the different parts of the antennal gland (Figures 6A and 6B). No immunoreactivity was detected in the coelomosac cells (Figure 6C). Na+,K+-ATPase was localized in the labyrinth and the bladder regions of the antennal glands (Figures 6D–6F). The cells of labyrinth I showed weak immunostaining (Figure 6E). A stronger immunoreaction was observed in the cells of labyrinth II (Figure 6F) and in those of the thick wall of the bladder (Figure 6D). In all immunostained cells, the basal region showed a much higher fluorescence than the apical side, and the nucleus was free of fluorescent marker (Figures 6D–6F).
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Discussion and Conclusion
To our knowledge, this study is the first ultrastructural investigation of the antennal glands of homarid lobsters. In contrast to the situation in freshwater species, no nephridial tubule was observed between the labyrinth and the bladder. In crayfish, the main function of the nephridial tubule is salt reabsorption, which results in the production of hyposmotic urine, a key adaptation to the low ion concentration of freshwater (Peterson and Loizzi 1974
; Sesma et al. 1983; Fuller et al. 1989; Khodabandeh et al. 2005a). This compartment is not functionally necessary in H. gammarus, a marine species that in seawater produces urine that is isotonic to hemolymph (Dall 1970).
The most striking features of the coelomosac cells (podocytes) are the presence of basal pedicles, large lateral channels, intracellular large dense bodies, Golgi complex, vesicles, and vacuoles. The cells possess also endocytic vesicles and residual bodies that become detached and pass (as apical globular vesicles) into the lumen of the coelomosac. They are similar to podocytes described in other species of crustaceans particularly in crayfish (Khodabandeh et al. 2005a). Their cytological features and previous investigations in crabs and crayfish have shown that the coelomosac cells perform an ultrafiltration and secretory activity similar to that of the vertebrate podocytes (Johnson 1980; Fuller et al. 1989; Khodabandeh et al. 2005a). The apical globular globules ("formed bodies" of Riegel 1966) are analogous to the dense bodies extruded by vertebrate podocytes. No Na+,K+-ATPase immunoreactivity was detected in the coelomosac cells, a result in agreement with the ultrastructure of these cells. Previous investigations regarding the activity of Na+,K+-ATPase in the antennal glands of Procambarus clracki and P. blandengi have shown that the coelomosac displays the lowest activity of Na+,K+-ATPase compared with the other parts of the antennal glands (Peterson and Loizzi 1975). In the crayfish Astacus leptodactylus, Na+,K+-ATPase immunoreactivity was also absent in the coelomosac cells (Khodabandeh et al. 2005b). These observations are thus in accordance with the involvement of the coelomosac podocytes in hemolymph ultrafiltration.
The general common features of the epithelial cells of the labyrinth and bladder as observed in the present study are apical microvilli, apical cytoplasmic extrusions, endocytosis vesicles, cytoplasmic vacuoles, and basal plasma membrane infoldings associated with mitochondria. In general, these characters suggest the existence of active ion transport, potentially associated with reabsorption, secretion, or uptake of materials such as glucose, amino acids, and small proteins, and reabsorption of solute and water, respectively. Morphological differences were observed between the different regions of labyrinth I and II. In labyrinth I, we observed two different cell types: columnar and cuboidal. They might be similar cells, but at different physiological states, as previously described in the labyrinth epithelium of the blue crab Callinectes sapidus (Johnson 1980). Columnar cells have irregular basal plasma membrane infoldings associated with mitochondria, intercellular spaces, irregular apical microvilli, lysosome-like bodies, apical globular vesicles containing cytoplasmic extrusions, and spherical excretory bodies (residual bodies). These characters suggest a flow of fluid through intercellular spaces and the secretion of spherical residual bodies, similar to those observed in the coelomosac cells. The apical cytoplasmic extrusions may also represent the final apocrine product of the activity of lysosomal enzymes. We conclude that these cells are potentially more active in secretory functions (maybe of nitrogenous wastes) than in reabsorption functions. This follows similar suggestions related to the function of labyrinth I cells in crayfish (Peterson and Loizzi 1974; Ischiguro 1975; Sesma et al. 1983; Fuller et al. 1989; Khodabandeh et al. 2005a) and of the proximal tubule of the vertebrate kidney (Kanli and Terreros 1997). One of the most important features of cuboidal cells is the association of endocytotic vesicles to apical microvilli, and the abundance of glycogen globules. These characters suggest that they are more active in the reabsorption of materials such as glucose or amino acids and maybe small proteins. The role of the labyrinth as an absorptive structure was proposed by Binns (1969), who suggested that the cells of the labyrinth in the crab Carcinus maenas might be important in the active reabsorption of glucose from the urine. More recent studies also strongly suggest a secretory and absorptive function for the labyrinth (Riegel and Cook 1975; Behnke et al. 1990), in particular for glucose (Burger 1957; Behnke et al. 1998). In Homarus, the cuboidal cells of labyrinth I could reabsorb the initial materials and store these materials, perhaps as glycogen, which is particularly abundant in these cells. The cuboidal cells with regular basal infoldings associated with numerous mitochondria, glycogen, and dense bodies linked to the presence of Na+,K+-ATPase, are also certainly involved in active ion transport, a classical role for the labyrinth cells in crustaceans (Riegel and Cook 1975; Fuller et al. 1989; Khodabandeh et al. 2005a,b). The large swollen mitochondria with small cristae could accumulate waste material. This hypothesis is reinforced by their association with lysosome-like bodies eliminated from the cells through apical extrusion. Mitochondria have been implicated in the accumulation and elimination of particles of different nature (e.g., heavy metals) in the labyrinth of crayfish (Miyawaki and Ukeshima 1967; Miyawaki and Ura 1969) or in the hepatopancreatic cells of the lobster (Chavez-Crooker et al. 2001,2002). Labyrinth II cells possess apical microvilli and basal plasma membrane infoldings associated with mitochondria and numerous large vacuoles. The Na+,K+-ATPase immunoactivity is strong in the basal portion of these cells. These features point to active ion transport and potential transepithelial fluid movement. As in labyrinth I cells, apical cytoplasmic extrusions suggest a secretory activity. The cells also possess large basal vacuoles containing fine particles, and they might pass through the cells into the lumen. These features suggest another secretory function for these cells.
The bladder cells present different sizes (15–50 µm) according to their location. Morphological similarities between the cells of labyrinth II and the bladder suggest functional analogies between these two sites. Because Na+,K+-ATPase was detected in the bladder cells, a last modification of the filtrate would occur in this region of the antennal gland before the release of urine. Investigations of the bladder cells in crayfish and crabs have shown that they are involved in active ion transport (Johnson 1980; Miller 1989; Khodabandeh et al. 2005a,b) or in the transport of solutes such as carbohydrates (Gross 1967; Holliday and Miller 1984; Sarver et al. 1994) and organic acids (Pritchard and Miller 1991). Compared with the labyrinth cells, the bladder cells present more developed basal infoldings and a denser network of vacuoles. Using the same antibody concentration, the immunoactivity of Na+,K+-ATPase was always more intense in the bladder than in the labyrinth, as in A. leptodactylus (Khodabandeh et al. 2005b). However, immunolabeling was more intense in the antennal gland of A. leptodactylus than in H. gammarus.
In conclusion, labyrinth and bladder cells are involved in different excretory and reabsorption functions. The presence of Na+,K+-ATPase in both structures could be explained in two ways: (a) the enzyme provides only the driving force for the transport of solutes such as sugar or amino acids or 2) the labyrinth and bladder cells are involved in active ion exchanges per se.
Lobsters can be found in coastal waters and estuarine habitats (Jury et al. 1994; Lawton and Lavalli 1995), where salinity fluctuates. At low salinity, they are able to slightly hyperosmoregulate (Charmantier et al. 1984) and to maintain their urine concentration slightly below the hemolymph osmolality by 20–50 mosm/kg (Dall 1970). This difference might originate from limited selective ion reabsorption through the labyrinth and bladder cells that present features of ionocytes including the presence of Na+,K+-ATPase. In conclusion, the antennal glands of adult H. gammarus, besides their implication in excretion, might participate in osmoregulation through active ion exchanges.
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Acknowledgments |
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Thanks are due to the University of Tarbiat Modarres and Ministry of Science, Research and Technology, Islamic Republic of Iran, for financial aid and support. Special thanks also to Dr. Khosrov Piri, responsible for Iranian student service in Paris, and F. Aujoulat, C. Blasco, E. Grousset, J.P. Selzner of the University of Montpellier II for their technical help.
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Footnotes |
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Received for publication September 29, 2004; accepted February 22, 2005
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