Supplemental Material - Schmitz et al. - Vol. 55, pp 515-524

Online Supplemental data for Schmitz et al.: Figures.

Files in this Data Supplement:

• Schmitz et al., Supplemental Figure 1 - Expression of CXCL15 (green) was evaluated by immunofluorescence in the three inflamed models of inflammation. The expression pattern in a mock stomach (A) and H. felis-infected stomach after eight weeks (B). Notice the increase of CXCL15 in the gastritis model. Figure 1C – F show CXCL15 expression in the colitis models. Figure 1C shows the expression in the FVB/N mouse colon and (D) shows the expression in the FVB.mdr1a^-/- colon at 5 months of age. The last two panels are colons from uninfected B6.Rag-1^-/- recipients (E) or H. hepaticus infected B6.Rag-1^-/- recipients (F) of CD4+ T cells from uninfected B6 mice. Note the slight decrease in CXCL15 expression in panel D and F. Bar = 50 microns.
• Schmitz et al., Supplemental Figure 2 - Immunofluorescent analysis of neutrophils in the gastritis and colitis models. A biotinylated rat-anti-mouse Gr-1 (Ly-6G) (clone RB6-8C5; BD Pharmingen, San Diego, CA; 0.1 µg/mL) was used to detect neutrophils. Visualization was by Streptavidin-HPR and Cy3-Tyramide. Figures 2 A and B show the expression of neutrophils in a mock-infected stomach (A) and a H. felis-infected stomach after eight weeks (B). Note the absence of neutrophils in panel A but an increase once the stomach becomes inflamed as seen in panel B. FVB/N (C) and FVB.mdr1a^-/- (D) colons showing the staining for neutrophils. Again notice the absence of neutrophils in the non-inflamed but the infiltration of neutrophils in the inflamed model. The last two panels show the expression of neutrophils in the uninfected B6.Rag-1^-/- recipients (E) or H. hepaticus infected B6.Rag-1^-/- recipients (F) of CD4+ T cells from uninfected B6 mice. Again notice the absence of neutrophils in the non-inflamed colon but an increase in the inflamed colon. Bar = 50 microns.