|
|
|
|
 |
|||
|
|||
ARTICLE |
Reduction of Lipofuscin-like Autofluorescence in Fluorescently Labeled Tissue
Stephen A. Schnella, William A. Stainesb, and Martin W. Wessendorfaa Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, Minnesota
b Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada
Correspondence to: Stephen A. Schnell, 4-102 Owre Hall, Dept. Cell Biology and Neuroanatomy, 321 Church Street SE, U. of Minnesota, Minneapolis, MN 55455.
The fluorescent pigment lipofuscin accumulates with age in the cytoplasm of cells of the CNS. Because of its broad excitation and emission spectra, the presence of lipofuscin-like autofluorescence complicates the use of fluorescence microscopy (e.g., fluorescent retrograde tract tracing and fluorescence immunocytochemistry). In this study we examined several chemical treatments of tissue sections for their ability to reduce or eliminate lipofuscin-like autofluorescence without adversely affecting other fluorescent labels. We found that 1–10 mM CuSO4 in 50 mM ammonium acetate buffer (pH 5) or 1% Sudan Black B (SB) in 70% ethanol reduced or eliminated lipofuscin autofluorescence in sections of monkey, human, or rat neural tissue. These treatments also slightly reduced the intensity of immunofluorescent labeling and fluorescent retrograde tract tracers. However, the reduction of these fluorophores was far less dramatic than that for the lipofuscin-like compound. We conclude that treatment of tissue with CuSO4 or SB provides a reasonable compromise between reduction of lipofuscin-like fluorescence and maintenance of specific fluorescent labels. (J Histochem Cytochem 47:719–730, 1999)
Key Words: copper sulfate, Sudan Black B, monkey, human, primate, rat, immunohistochemistry, immunocytochemistry, retrograde axonal tract tracing
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Schmidtko, W. Gao, P. Konig, S. Heine, R. Motterlini, P. Ruth, J. Schlossmann, D. Koesling, E. Niederberger, I. Tegeder, et al. cGMP Produced by NO-Sensitive Guanylyl Cyclase Essentially Contributes to Inflammatory and Neuropathic Pain by Using Targets Different from cGMP-Dependent Protein Kinase I J. Neurosci., August 20, 2008; 28(34): 8568 - 8576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Nakamura, H. Kameda, Y. Koshimizu, Y. Yanagawa, and T. Kaneko Production and Histological Application of Affinity-purified Antibodies to Heat-denatured Green Fluorescent Protein J. Histochem. Cytochem., July 1, 2008; 56(7): 647 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Igaz, L. K. Kwong, Y. Xu, A. C. Truax, K. Uryu, M. Neumann, C. M. Clark, L. B. Elman, B. L. Miller, M. Grossman, et al. Enrichment of C-Terminal Fragments in TAR DNA-Binding Protein-43 Cytoplasmic Inclusions in Brain but not in Spinal Cord of Frontotemporal Lobar Degeneration and Amyotrophic Lateral Sclerosis Am. J. Pathol., July 1, 2008; 173(1): 182 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hadziahmetovic, T. Dentchev, Y. Song, N. Haddad, X. He, P. Hahn, D. Pratico, R. Wen, Z. L. Harris, J. D. Lambris, et al. Ceruloplasmin/Hephaestin Knockout Mice Model Morphologic and Molecular Features of AMD Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2728 - 2736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schmidtko, W. Gao, M. Sausbier, I. Rauhmeier, U. Sausbier, E. Niederberger, K. Scholich, A. Huber, W. Neuhuber, H.-D. Allescher, et al. Cysteine-Rich Protein 2, a Novel Downstream Effector of cGMP/cGMP-Dependent Protein Kinase I-Mediated Persistent Inflammatory Pain J. Neurosci., February 6, 2008; 28(6): 1320 - 1330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Zeis, U. Graumann, R. Reynolds, and N. Schaeren-Wiemers Normal-appearing white matter in multiple sclerosis is in a subtle balance between inflammation and neuroprotection Brain, January 1, 2008; 131(1): 288 - 303. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Bentzon, C. S. Sondergaard, M. Kassem, and E. Falk Smooth Muscle Cells Healing Atherosclerotic Plaque Disruptions Are of Local, Not Blood, Origin in Apolipoprotein E Knockout Mice Circulation, October 30, 2007; 116(18): 2053 - 2061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Whitman and C. A. Greer Synaptic Integration of Adult-Generated Olfactory Bulb Granule Cells: Basal Axodendritic Centrifugal Input Precedes Apical Dendrodendritic Local Circuits J. Neurosci., September 12, 2007; 27(37): 9951 - 9961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Mandell, L. Berglin, E. A. Severson, H. F. Edelhauser, and C. A. Parkos Expression of JAM-A in the Human Corneal Endothelium and Retinal Pigment Epithelium: Localization and Evidence for Role in Barrier Function Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 3928 - 3936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Szabadits, C. Cserep, A. Ludanyi, I. Katona, J. Gracia-Llanes, T. F. Freund, and G. Nyiri Hippocampal GABAergic Synapses Possess the Molecular Machinery for Retrograde Nitric Oxide Signaling J. Neurosci., July 25, 2007; 27(30): 8101 - 8111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Uchida, S.-i. Nakano, F. Gomi, and H. Takahashi Differential Regulation of Basic Helix-Loop-Helix Factors Mash1 and Olig2 by beta-Amyloid Accelerates Both Differentiation and Death of Cultured Neural Stem/Progenitor Cells J. Biol. Chem., July 6, 2007; 282(27): 19700 - 19709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L Herington and B. M Bany The conceptus increases secreted phosphoprotein 1 gene expression in the mouse uterus during the progression of decidualization mainly due to its effects on uterine natural killer cells Reproduction, June 1, 2007; 133(6): 1213 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Benito, J. P. Romero, R. M. Tolon, D. Clemente, F. Docagne, C. J. Hillard, C. Guaza, and J. Romero Cannabinoid CB1 and CB2 Receptors and Fatty Acid Amide Hydrolase Are Specific Markers of Plaque Cell Subtypes in Human Multiple Sclerosis J. Neurosci., February 28, 2007; 27(9): 2396 - 2402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Feng, M. C. Jaimes, N. H. Lazarus, D. Monak, C. Zhang, E. C. Butcher, and H. B. Greenberg Redundant Role of Chemokines CCL25/TECK and CCL28/MEC in IgA+ Plasmablast Recruitment to the Intestinal Lamina Propria After Rotavirus Infection J. Immunol., May 15, 2006; 176(10): 5749 - 5759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tande, G. Hoglinger, T. Debeir, N. Freundlieb, E. C. Hirsch, and C. Francois New striatal dopamine neurons in MPTP-treated macaques result from a phenotypic shift and not neurogenesis Brain, May 1, 2006; 129(5): 1194 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Uryu, C. Richter-Landsberg, W. Welch, E. Sun, O. Goldbaum, E. H. Norris, C.-T. Pham, I. Yazawa, K. Hilburger, M. Micsenyi, et al. Convergence of Heat Shock Protein 90 with Ubiquitin in Filamentous {alpha}-Synuclein Inclusions of {alpha}-Synucleinopathies Am. J. Pathol., March 1, 2006; 168(3): 947 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Ngwenya, A. Peters, and D. L. Rosene Light and Electron Microscopic Immunohistochemical Detection of Bromodeoxyuridine-labeled Cells in the Brain: Different Fixation and Processing Protocols J. Histochem. Cytochem., July 1, 2005; 53(7): 821 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Harkany, C. Holmgren, W. Hartig, T. Qureshi, F. A. Chaudhry, J. Storm-Mathisen, M. B. Dobszay, P. Berghuis, G. Schulte, K. M. Sousa, et al. Endocannabinoid-Independent Retrograde Signaling at Inhibitory Synapses in Layer 2/3 of Neocortex: Involvement of Vesicular Glutamate Transporter 3 J. Neurosci., May 26, 2004; 24(21): 4978 - 4988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Zainelli, C. A. Ross, J. C. Troncoso, J. K. Fitzgerald, and N. A. Muma Calmodulin Regulates Transglutaminase 2 Cross-Linking of Huntingtin J. Neurosci., February 25, 2004; 24(8): 1954 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Veldic, H. J. Caruncho, W. S. Liu, J. Davis, R. Satta, D. R. Grayson, A. Guidotti, and E. Costa DNA-methyltransferase 1 mRNA is selectively overexpressed in telencephalic GABAergic interneurons of schizophrenia brains PNAS, January 6, 2004; 101(1): 348 - 353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Araki, S. Tomita, H. Yamaguchi, N. Miyagi, A. Sumioka, Y. Kirino, and T. Suzuki Novel Cadherin-related Membrane Proteins, Alcadeins, Enhance the X11-like Protein-mediated Stabilization of Amyloid {beta}-Protein Precursor Metabolism J. Biol. Chem., December 5, 2003; 278(49): 49448 - 49458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bresjanac, L. M. Smid, T. D. Vovko, A. Petric, J. R. Barrio, and M. Popovic Molecular-Imaging Probe 2-(1-{6-[(2-Fluoroethyl)(Methyl) Amino]-2-Naphthyl}Ethylidene) Malononitrile Labels Prion Plaques In Vitro J. Neurosci., September 3, 2003; 23(22): 8029 - 8033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cremer, K. Kupper, B. Wagler, L. Wizelman, J. v. Hase, Y. Weiland, L. Kreja, J. Diebold, M. R. Speicher, and T. Cremer Inheritance of gene density-related higher order chromatin arrangements in normal and tumor cell nuclei J. Cell Biol., September 1, 2003; 162(5): 809 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mezey, S. Key, G. Vogelsang, I. Szalayova, G. D. Lange, and B. Crain Transplanted bone marrow generates new neurons in human brains PNAS, February 4, 2003; 100(3): 1364 - 1369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Virgintino, D. Robertson, M. Errede, V. Benagiano, F. Girolamo, E. Maiorano, L. Roncali, and M. Bertossi Expression of P-Glycoprotein in Human Cerebral Cortex Microvessels J. Histochem. Cytochem., December 1, 2002; 50(12): 1671 - 1676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Konishi, K. Lindholm, L.-B. Yang, R. Li, and Y. Shen Isolation of Living Neurons from Human Elderly Brains Using the Immunomagnetic Sorting DNA-Linker System Am. J. Pathol., November 1, 2002; 161(5): 1567 - 1576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Modregger, N. A. DiProspero, V. Charles, D. A. Tagle, and M. Plomann PACSIN 1 interacts with huntingtin and is absent from synaptic varicosities in presymptomatic Huntington's disease brains Hum. Mol. Genet., October 2, 2002; 11(21): 2547 - 2558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Williams, A. Schwartz, S. Corey, M. Orandle, W. Kennedy, B. Thompson, X. Alvarez, C. Brown, S. Gartner, and A. Lackner Proliferating Cellular Nuclear Antigen Expression as a Marker of Perivascular Macrophages in Simian Immunodeficiency Virus Encephalitis Am. J. Pathol., August 1, 2002; 161(2): 575 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. Ananyeva, D. V. Kouiavskaia, M. Shima, and E. L. Saenko Intrinsic pathway of blood coagulation contributes to thrombogenicity of atherosclerotic plaque Blood, May 29, 2002; 99(12): 4475 - 4485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Neumann and D. Gabel Simple Method for Reduction of Autofluorescence in Fluorescence Microscopy J. Histochem. Cytochem., March 1, 2002; 50(3): 437 - 439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang, A. S. Basile, J. Gomeza, L. A. Volpicelli, A. I. Levey, and J. Wess Characterization of Central Inhibitory Muscarinic Autoreceptors by the Use of Muscarinic Acetylcholine Receptor Knock-Out Mice J. Neurosci., March 1, 2002; 22(5): 1709 - 1717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Baschong, R. Suetterlin, and R. H. Laeng Control of Autofluorescence of Archival Formaldehyde-fixed, Paraffin-embedded Tissue in Confocal Laser Scanning Microscopy (CLSM) J. Histochem. Cytochem., December 1, 2001; 49(12): 1565 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Tsai, R. L. van Bendegem, S. W. Hwang, and C. H. Tator A Novel Method for Simultaneous Anterograde and Retrograde Labeling of Spinal Cord Motor Tracts in the Same Animal J. Histochem. Cytochem., September 1, 2001; 49(9): 1111 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Delatour, L. Mercken, K. H. El Hachimi, M.-A. Colle, L. Pradier, and C. Duyckaerts FE65 in Alzheimer's Disease : Neuronal Distribution and Association with Neurofibrillary Tangles Am. J. Pathol., May 1, 2001; 158(5): 1585 - 1591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Williams, S. Corey, S. V. Westmoreland, D. Pauley, H. Knight, C. deBakker, X. Alvarez, and A. A. Lackner Perivascular Macrophages Are the Primary Cell Type Productively Infected by Simian Immunodeficiency Virus in the Brains of Macaques: Implications for the Neuropathogenesis of AIDS J. Exp. Med., April 9, 2001; 193(8): 905 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miyasaka, M. Morishima-Kawashima, R. Ravid, P. Heutink, J. C. van Swieten, K. Nagashima, and Y. Ihara Molecular Analysis of Mutant and Wild-Type Tau Deposited in the Brain Affected by the FTDP-17 R406W Mutation Am. J. Pathol., February 1, 2001; 158(2): 373 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Agdeppa, V. Kepe, J. Liu, S. Flores-Torres, N. Satyamurthy, A. Petric, G. M. Cole, G. W. Small, S.-C. Huang, and J. R. Barrio Binding Characteristics of Radiofluorinated 6-Dialkylamino-2-Naphthylethylidene Derivatives as Positron Emission Tomography Imaging Probes for beta -Amyloid Plaques in Alzheimer's Disease J. Neurosci., December 15, 2001; 21(24): RC189 - RC189. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |