|
|
|
|
 |
|||
|
|||
ARTICLE |
Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Antibodies to Cleaved Caspase 3
Allen M. Gowna and Mark C. Willinghamba PhenoPath Laboratories and IRIS, Seattle, Washington
b Department of Pathology, Wake Forest University School of Medicine, Winston–Salem, North Carolina
Correspondence to: Mark C. Willingham, Dept. of Pathology, Wake Forest University School of Medicine, Winston–Salem, NC 27157–1072. E-mail: mwilling@wfubmc.edu
Apoptosis has gained central importance in the study of many biological processes, including neoplasia, neurodegenerative diseases, and development. One of the limitations of many studies is the difficulty of specifically identifying individual apoptotic cells. Of the many specific methods developed to detect apoptotic cells, most are not applicable to histological sections of archival paraffin-embedded tissues. Recently, advances in the understanding of the molecular events in apoptosis have led to the realization that caspase activation is by far the most specific indicator of this cell suicide mechanism. Several publications have reported the development of antibodies directed at neoepitopes that are generated in various substrates through the action of caspases. One of these is that present on activated caspase 3, a ubiquitously distributed caspase that is a main effector caspase of the apoptotic cascade within cells. This study demonstrates the utility of using a recently commercially available antibody to cleaved caspase 3 in archival paraffin sections, suggesting that this may be a highly specific and sensitive method generally applicable to many studies of archival material. (J Histochem Cytochem 50:449–454, 2002)
Key Words: apoptosis, caspase, immunohistochemistry, TNF, ricin
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Abd-Elrahman, K. Hershko, T. Neuman, B. Nachmias, R. Perlman, and D. Ben-Yehuda The Inhibitor of Apoptosis Protein Livin (ML-IAP) Plays a Dual Role in Tumorigenicity Cancer Res., July 1, 2009; 69(13): 5475 - 5480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. West, E. D.J. Courtney, A. P. Poullis, and R. J. Leicester Apoptosis in the Colonic Crypt, Colorectal Adenomata, and Manipulation by Chemoprevention Cancer Epidemiol. Biomarkers Prev., June 1, 2009; 18(6): 1680 - 1687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bressenot, S. Marchal, L. Bezdetnaya, J. Garrier, F. Guillemin, and F. Plenat Assessment of Apoptosis by Immunohistochemistry to Active Caspase-3, Active Caspase-7, or Cleaved PARP in Monolayer Cells and Spheroid and Subcutaneous Xenografts of Human Carcinoma J. Histochem. Cytochem., April 1, 2009; 57(4): 289 - 300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M G Gnanalingham, P Williams, V Wilson, J Bispham, M A Hyatt, A Pellicano, H Budge, T Stephenson, and M E Symonds Nutritional manipulation between early to mid-gestation: effects on uncoupling protein-2, glucocorticoid sensitivity, IGF-I receptor and cell proliferation but not apoptosis in the ovine placenta Reproduction, October 1, 2007; 134(4): 615 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Seeliger, M. l. Brugmann, L. Kruger, I. Greiser-Wilke, J. Verspohl, J. Segales, and W. Baumgartner Porcine Circovirus Type 2-Associated Cerebellar Vasculitis in Postweaning Multisystemic Wasting Syndrome (PMWS)-Affected Pigs Vet. Pathol., September 1, 2007; 44(5): 621 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kobayashi, J. Masumoto, T. Tada, T. Nomiyama, K. Hongo, and J. Nakayama Prognostic Significance of the Immunohistochemical Staining of Cleaved Caspase-3, an Activated Form of Caspase-3, in Gliomas Clin. Cancer Res., July 1, 2007; 13(13): 3868 - 3874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Panjrath, V. Patel, C. I. Valdiviezo, N. Narula, J. Narula, and D. Jain Potentiation of Doxorubicin Cardiotoxicity by Iron Loading in a Rodent Model J. Am. Coll. Cardiol., June 26, 2007; 49(25): 2457 - 2464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. McClusky, C de Jager, and M. Bornman Stage-Related Increase in the Proportion of Apoptotic Germ Cells and Altered Frequencies of Stages in the Spermatogenic Cycle Following Gestational, Lactational, and Direct Exposure of Male Rats to p-Nonylphenol Toxicol. Sci., January 1, 2007; 95(1): 249 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Halpern, J. A. Clark, T. A. Saunders, S. M. Doelle, D. M. Hosseini, A. M. Stagner, and B. Dvorak Reduction of experimental necrotizing enterocolitis with anti-TNF-{alpha} Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G757 - G764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S A Khan, D Bhandare, and R T Chatterton Jr The local hormonal environment and related biomarkers in the normal breast Endocr. Relat. Cancer, September 1, 2005; 12(3): 497 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Holubec, C. M. Payne, H. Bernstein, K. Dvorakova, C. Bernstein, C. N. Waltmire, J. A. Warneke, and H. Garewal Assessment of Apoptosis by Immunohistochemical Markers Compared to Cellular Morphology in Ex Vivo-stressed Colonic Mucosa J. Histochem. Cytochem., February 1, 2005; 53(2): 229 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Piazza, J. A. Costoya, T. Merghoub, R. M. Hobbs, and P. P. Pandolfi Disruption of PLZP in Mice Leads to Increased T-Lymphocyte Proliferation, Cytokine Production, and Altered Hematopoietic Stem Cell Homeostasis Mol. Cell. Biol., December 1, 2004; 24(23): 10456 - 10469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Westcott, R. J. Abi-Habib, K. A. Cohen, M. C. Willingham, S. Liu, T. H. Bugge, S. H. Leppla, and A. E. Frankel Diphtheria toxin-murine granulocyte-macrophage colony-stimulating factor-induced hepatotoxicity is mediated by Kupffer cells Mol. Cancer Ther., December 1, 2004; 3(12): 1681 - 1689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Welsh, R. Williams, L. Kirkpatrick, G. Paine-Murrieta, and G. Powis Antitumor activity and pharmacodynamic properties of PX-478, an inhibitor of hypoxia-inducible factor-1{alpha} Mol. Cancer Ther., March 1, 2004; 3(3): 233 - 244. [Abstract] [Full Text]
|
|
|
|
|
|
D. J. Brat, A. A. Castellano-Sanchez, S. B. Hunter, M. Pecot, C. Cohen, E. H. Hammond, S. N. Devi, B. Kaur, and E. G. Van Meir Pseudopalisades in Glioblastoma Are Hypoxic, Express Extracellular Matrix Proteases, and Are Formed by an Actively Migrating Cell Population Cancer Res., February 1, 2004; 64(3): 920 - 927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V.-S. Eckle, A. Buchmann, W. Bursch, R. Schulte-Hermann, and M. Schwarz Immunohistochemical Detection of Activated Caspases in Apoptotic Hepatocytes in Rat Liver Toxicol Pathol, January 1, 2004; 32(1): 9 - 15. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Chopin, R. Barei-Moniri, P. Maille, M.-A. Le Frere-Belda, B. Muscatelli-Groux, N. Merendino, L. Lecerf, A. Stoppacciaro, and F. Velotti Human Urinary Bladder Transitional Cell Carcinomas Acquire the Functional Fas Ligand during Tumor Progression Am. J. Pathol., April 1, 2003; 162(4): 1139 - 1149. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |
