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Originally published as JHC exPRESS on July 11, 2005.
doi:10.1369/jhc.5C6703.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (10): 1199-1202, 2005
Copyright ©The Histochemical Society, Inc.

RAPID COMMUNICATION

An Alternative Pretreatment Procedure in Animal Transmissible Spongiform Encephalopathies Diagnosis Using PrPsc Immunohistochemistry

Anna A. Bencsik, Sabine O.S. Debeer and Thierry G.M. Baron

Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité Agent Transmissible Non Conventionnel (ATNC), Lyon, France

Correspondence to: Dr. Anna Bencsik, AFSSA, Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité ATNC, 31 Avenue Tony Garnier, 69364 Lyon, Cedex 07-France. E-mail: a.bencsik{at}lyon.afssa.fr

Because of its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used more often in the diagnosis of transmissible spongiform encephalopathies (TSEs), such as scrapie and bovine spongiform encephalopathy (BSE). PrPsc IHC requires a combination of pretreatments (chemical, heating, and enzymatic). The method of application may depend on the anti-prion antibody considered. If these pretreatments are efficient for diagnostic purpose, it may, however, be interesting to use an alternative method to efficiently detect PrPsc IHC immunohistochemically using chemical pretreatments solely. Here we describe such pretreatments reporting the difficulty (section adhesion) but also the potential advantages of such methods (easy, quick, inexpensive, and amplifying effect). (J Histochem Cytochem 53:1199–1202, 2005)

Key Words: bovine spongiform • encephalopathy • diagnosis • antigen retrieval • immunohistochemistry • prion • scrapie


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A. A. Bencsik, A. W. Coleman, S. O.S. Debeer, H. Perron, and A. Moussa
Amplified Immunohistochemical Detection of PrPsc in Animal Transmissible Spongiform Encephalopathies Using Streptomycin
J. Histochem. Cytochem., August 1, 2006; 54(8): 849 - 853.
[Abstract] [Full Text] [PDF]



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