Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
Originally published as JHC exPRESS on January 6, 2006.
doi:10.1369/jhc.5B6851.2006
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jhc.5B6851.2006v1
54/6/739    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shi, S.-R.
Right arrow Articles by Taylor, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shi, S.-R.
Right arrow Articles by Taylor, C. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 54 (6): 739-743, 2006
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Protein Extraction from Formalin-fixed, Paraffin-embedded Tissue Sections: Quality Evaluation by Mass Spectrometry

Shan-Rong Shi, Cheng Liu, Brian M. Balgley, Cheng Lee and Clive R. Taylor

Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California (S-RS,CLiu,CRT), and Calibrant Biosystems, Gaithersburg, Maryland (BMB,Clee)

Correspondence to: Clive R. Taylor, MD, PhD, Department of Pathology, University of Southern California Keck School of Medicine HMR 204, 2011 Zonal Avenue, Los Angeles, CA 90033. E-mail: taylor{at}pathfinder.hsc.usc.edu

A satisfactory protocol of protein extraction has been established based on the heat-induced antigen retrieval (AR) technique widely applied in immunohistochemistry for archival formalin-fixed, paraffin-embedded (FFPE) tissue sections. Based on AR, an initial serial experiment to identify an optimal protocol of heat-induced protein extraction was carried out using FFPE mouse tissues. The optimal protocol for extraction of proteins was then performed on an archival FFPE tissue of human renal carcinoma. FFPE sections were boiled in a retrieval solution of Tris-HCl containing 2% SDS, followed by incubation. Fresh tissue taken from the same case of renal carcinoma was processed for extraction of proteins by a conventional method using radioimmunoprecipitation assay solution, to compare the efficiency of protein extraction from FFPE tissue sections with extraction from fresh tissue. As a control, further sections of the same FFPE sample were processed by the same procedure without heating treatment. Evaluation of the quality of protein extracted from FFPE tissue was done using gel electrophoresis and mass spectrometry, showing most identified proteins extracted from FFPE tissue sections were overlapped with those extracted from fresh tissue. (J Histochem Cytochem 54:739–743, 2006)

Key Words: protein extraction • antigen retrieval • formalin-fixed • paraffin-embedded tissue • mass spectrometry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Brief Funct Genomic ProteomicHome page
M. Asslaber and K. Zatloukal
Biobanks: transnational, European and global networks
Brief Funct Genomic Proteomic, October 4, 2007; (2007) elm023v1.
[Abstract] [Full Text] [PDF]

Home page
 jvdiHome page
E. M. Nicholson, R. A. Kunkle, A. N. Hamir, S. Lebepe-Mazur, and D. Orcutt
Detection of the disease-associated isoform of the prion protein in formalin-fixed tissues by Western blot
J Vet Diagn Invest, September 1, 2007; 19(5): 548 - 552.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. P. Levy, K. R. Purushothaman, N. S. Levy, M. Purushothaman, M. Strauss, R. Asleh, S. Marsh, O. Cohen, S. K. Moestrup, H. J. Moller, et al.
Downregulation of the Hemoglobin Scavenger Receptor in Individuals With Diabetes and the Hp 2-2 Genotype: Implications for the Response to Intraplaque Hemorrhage and Plaque Vulnerability
Circ. Res., July 6, 2007; 101(1): 106 - 110.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
T. Guo, W. Wang, P. A. Rudnick, T. Song, J. Li, Z. Zhuang, R. J. Weil, D. L. DeVoe, C. S. Lee, and B. M. Balgley
Proteome Analysis of Microdissected Formalin-fixed and Paraffin-embedded Tissue Specimens
J. Histochem. Cytochem., July 1, 2007; 55(7): 763 - 772.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2006