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Originally published as JHC exPRESS on July 11, 2007.
doi:10.1369/jhc.7A7273.2007
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Journal of Histochemistry and Cytochemistry
Volume 55 (11): 1129-1137, 2007
Copyright ©The Histochemical Society, Inc.

Mitochondrial Ferritin Expression in Adult Mouse Tissues

Paolo Santambrogio, Giorgio Biasiotto, Francesca Sanvito, Stefano Olivieri, Paolo Arosio and Sonia Levi

Department of Bio Technology (SL,PS), Department of Pathology (FS,SO), San Raffaele Scientific Institute, Milan, Italy; Section of Chemistry, Faculty of Medicine, University of Brescia, Brescia, Italy (GB,PA); and Vita-Salute, San Raffaele University, Milan, Italy (SL)

Correspondence to: Prof. Sonia Levi, Vita-Salute, San Raffaele University and San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy. E-mail: levi.sonia{at}hsr.it

Mitochondrial ferritin (FtMt) is a novel ferritin type specifically targeted to mitochondria. It is highly expressed in the human testis and in sideroblasts from patients with sideroblastic anemia, but other organs have not been studied. To study its expression in the main organs of the mouse, we first used RT-PCR and then produced recombinant mouse FtMt and specific antibodies. Immunohistochemistry analyses confirmed that FtMt is highly expressed in mouse testis, particularly in spermatocytes and interstitial Leydig cells. The protein was also identified in other organs including heart, brain, spinal cord, kidney, and pancreatic islet of Langerhans but not in liver and splenocytes, which have iron storage function and express high levels of cytosolic ferritins. Results indicate that the primary function of ferritin FtMt is not involved in storing cellular or body iron, but its association with cell types characterized by high metabolic activity and oxygen consumption suggests a role in protecting mitochondria from iron-dependent oxidative damage. (J Histochem Cytochem 55:1129–1137, 2007)

Key Words: mitochondrial ferritin • expression • iron • immunohistochemistry


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