This Article Full Text Full Text (PDF) All Versions of this Article: jhc.7A7321.2007v1 56/4/329    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Zhu, S. Articles by Litvin, J. PubMed PubMed Citation Articles by Zhu, S. Articles by Litvin, J. Social Bookmarking                      What's this?

Immunolocalization of Periostin-like Factor and Periostin During Embryogenesis

Shimei Zhu, Mary F. Barbe¹, Neilay Amin, Shobha Rani, Steven N. Popoff, Fayez F. Safadi and Judith Litvin¹

Department of Anatomy and Cell Biology (SZ,MFB,NA,SR,SNP,FFS,JL), Musculoskeletal Research Group (MFB,SNP,FFS,JL), and Department of Physical Therapy, College of Health Professions (MFB), Temple Medical School, Philadelphia, Pennsylvania

Correspondence to: Judith Litvin, PhD, Department of Anatomy and Cell Biology, Temple Medical School, 3420 N. Broad Street, MRB 615, Philadelphia, PA 19140. E-mail: Judith.litvin{at}temple.edu

Periostin-like factor (PLF) and Periostin are alternatively spliced mRNAs. Our findings are the first to show similarities and differences between PLF and Periostin location using isoform-specific antibodies. The differences in when and where they are present during mouse embryogenesis suggest that they may have different functions. Using immunostaining techniques, we observed that PLF was highly expressed at 12.5 days postconception (dpc) in the intermediate and outer zones of most brain regions, spinal cord, cranial and spinal nerves, and chondrocytes in developing bone and in the heart wall. By 16.5 dpc, PLF was also present in ameloblasts and odontoblasts in developing teeth, and by 19.5 dpc, PLF was present at low levels only in vagal nerve bundles, discrete white matter bundles in the brain, and chondrocytes of developing ribs. Periostin, on the other hand, was absent at 12.5 dpc from dorsal spinal cord and from cranial and spinal nerves. By 16.5 dpc, Periostin was present in many spinal nerves, but absent thereafter, and at 19.5 dpc, Periostin was present in chondrocytes in developing bone but not in neural tissues. The different spatial and temporal location of PLF and Periostin in cartilage and bone cells suggests different roles for these proteins in endochondral bone formation. The early expression of PLF in brain differentiation zones and in developing axon bundles and nerves suggests that it may facilitate axon growth. (J Histochem Cytochem 56:329–345, 2008)

Key Words: Periostin-like factor • embryogenesis • heart • brain • spinal cord • nerves

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2008