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Originally published as JHC exPRESS on December 10, 2007.
doi:10.1369/jhc.7A7287.2007
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Journal of Histochemistry and Cytochemistry
Volume 56 (4): 347-358, 2008
Copyright ©The Histochemical Society, Inc.

An Immunohistochemical Method for Identifying Fibroblasts in Formalin-fixed, Paraffin-embedded Tissue

Tracy Goodpaster, Aster Legesse-Miller, Meera R. Hameed, Seena C. Aisner, Julie Randolph-Habecker and Hilary A. Coller

Experimental Histopathology, Fred Hutchinson Cancer Research Center, Seattle, Washington (TG,JR-H); Department of Molecular Biology, Princeton University, Princeton, New Jersey (AL-M,HAC); and Department of Pathology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey (MRH,SCA)

Correspondence to: Hilary A. Coller, PhD, Department of Molecular Biology, Lewis Thomas Laboratory, Room 140, Princeton University, Princeton, NJ 08544. E-mail: hcoller{at}princeton.edu

Fibroblasts are critical for tissue homeostasis, and their inappropriate proliferation and activation can result in common and debilitating conditions including fibrosis and cancer. We currently have a poor understanding of the mechanisms that control the growth and activation of fibroblasts in vivo, in part because of a lack of suitable fibroblast markers. We have taken advantage of an antibody previously shown to stain stromal cells in frozen tissues (TE-7) and identified conditions in which it can be used to stain fibroblasts and myofibroblasts in the paraffin-embedded tissue samples routinely collected for pathological analysis. We show that this antibody recognizes growing and quiescent fibroblasts and myofibroblasts by immunohistochemistry, immunofluorescence, and ELISA assays. We also present its staining patterns in normal tissue samples and in breast tumors. (J Histochem Cytochem 56:347–358, 2008)

Key Words: fibroblasts • immunohistochemistry • TE-7


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