Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

Originally published as JHC exPRESS on September 28, 2009.
doi:10.1369/jhc.2009.954586
Journal of Histochemistry and Cytochemistry
Volume 58 (2): 109-118, 2010
Copyright © 2010 van der Loos et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jhc.2009.954586v1
58/2/109    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by van der Loos, C. M.
Right arrow Articles by van der Wal, A. C.
PubMed
Right arrow PubMed Citation
Right arrow Articles by van der Loos, C. M.
Right arrow Articles by van der Wal, A. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Anti-human Vascular Endothelial Growth Factor (VEGF) Antibody Selection for Immunohistochemical Staining of Proliferating Blood Vessels

Chris M. van der Loos, Lorine B. Meijer-Jorna, Marloes E.C. Broekmans, Hanneke P.H.M. Ploegmakers, Peter Teeling, Onno J. de Boer and Allard C. van der Wal

Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands

Correspondence to: Chris M. van der Loos, PhD, Academic Medical Center, Department of Pathology, M2-230, Meibergdreef 9, NL-1105, AZ, Amsterdam, The Netherlands. E-mail: c.m.vanderloos{at}amc.uva.nl

Nine commercially available vascular endothelial growth factor (VEGF) antibodies were investigated for their ability to immunostain vascular malformations (VMs) with or without immature capillary proliferation. First, all antibodies were optimized for their performance in IHC, with placenta and colon adenocarcinoma as positive control tissues. Five antibodies were regarded as unfit for VEGF immunostaining based on poor immunostaining criteria. Subsequently, Western blot analysis using VEGF rabbit polyclonal antibody (Thermo RB-9031) revealed a clear 45-kDa band in tissue extracts from VMs with immature capillary proliferation and a high Ki67-labeling index, whereas tissue extracts from mature VMs without microvascular proliferation and no Ki67-labeling index demonstrated only a very weak 45-kDa band. In contrast, two VEGF antibodies, including the popular Santa Cruz A-20, revealed bands at 45 kDa of similar intensity in tissue extracts from both types of VMs. Staining characteristics of the 45-kDa band were reflected in the results obtained in IHC. (J Histochem Cytochem 58:109–118, 2010)

Key Words: placenta • colon cancer • endothelium • VEGF • immunohistochemistry • angiogenesis


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2010

 
Purchase HCS Short Course Manual on HCS site