Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
JHC exPRESS: First Published February 18, 2008. doi:10.1369/jhc.2008.950592
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on May 1, 2008.
This Article
Right arrow exPRESS PDF
Right arrow All Versions of this Article:
jhc.2008.950592v1
56/5/495    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bothe, M. K.
Right arrow Articles by Gruber, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bothe, M. K.
Right arrow Articles by Gruber, A. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Articles

The Murine mCLCA6 Is an Integral Apical Membrane Protein of Non-goblet Cell Enterocytes and Co-localizes With the Cystic Fibrosis Transmembrane Conductance Regulator

Melanie K. Bothe 1, Josephine Braun 1, Lars Mundhenk 1 and Achim D. Gruber 1*

1 Department of Veterinary Pathology, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany

* To whom correspondence should be addressed. E-mail: gruber.achim{at}vetmed.fu-berlin.de.

Submitted on December 16, 2007
Accepted on 30 January 2008


  Abstract
The CLCA family of proteins consists of a growing number of structurally and functionally diverse members with distinct expression patterns in different tissues. Several CLCA homologues have been implicated in diseases with secretory dysfunctions in the respiratory and intestinal tracts. Here, biochemical protein characterization and details on the cellular and subcellular expression pattern of the murine mCLCA6 are presented using specific antibodies directed against the amino- and carboxy-terminal cleavage products of mCLCA6. Computational and biochemical characterizations revealed protein processing and structural elements shared with hCLCA2, including anchorage in the apical cell membrane by a transmembrane domain in the carboxy-terminal subunit. A systematic light and electron microscopic immunolocalization found mCLCA6 to be associated with the microvilli of non-goblet cell enterocytes in the murine small and large intestine but no other tissues. The expression pattern was confirmed by quantitative RT-PCR following laser capture microdissection of relevant tissues. Confocal laser scanning microscopy co-localized the mCLCA6 protein with the cystic fibrosis transmembrane conductance regulator CFTR at the apical surface of colonic crypt cells. Together with previously published functional data, the results support a direct or indirect role of mCLCA6 in transepithelial anion conductance in the mouse intestine.

Key Words: calcium-activated chloride channel, mCLCA6, non-goblet cell enterocytes, mouse, intestine, cystic fibrosis, CFTR, membrane protein


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2008