Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

JHC exPRESS: First Published May 27, 2008. doi:10.1369/jhc.2008.950790
Journal of Histochemistry and Cytochemistry
Copyright © 2008 O’Donnell et al.

A more recent version of this article appeared on September 1, 2008.
This Article
Right arrow exPRESS PDF
Right arrow All Versions of this Article:
jhc.2008.950790v1
56/9/803    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O’Donnell, R. K.
Right arrow Articles by Muschel, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O’Donnell, R. K.
Right arrow Articles by Muschel, R. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Articles

Immunohistochemical Method Identifies Lymphovascular Invasion in a Majority of Oral Squamous Cell Carcinomas and Discriminates Between Blood and Lymphatic Vessel Invasion

Rebekah K. O’Donnell 1*, Michael Feldman 1, Rosemarie Mick 1 and Ruth J. Muschel 1

1 Department of Pathology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania (RKO,RJM); Department of Pathology, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania (MF); and Department of Biostatistics and Epidemiology, University of Pennsylvania, Philadelphia, Pennsylvania (RM)

* To whom correspondence should be addressed. E-mail: rebekah.k.odonnell{at}gmail.com.

Submitted on January 20, 2008
Accepted on 5 May 2008


  Abstract
Tumor invasion into blood and/or lymphatic channels is an important component of cancer staging and prognosis. Standard pathological methods do not provide sufficient contrast to discriminate between invasion into each type of vessel and are complicated by tissue retraction artifacts. We evaluated the ability of a triple-stain immunohistochemical method, combining cytokeratin, CD34, and podoplanin stains in a single section, to distinguish blood from lymphatic vascular invasion in oral squamous cell carcinoma and confirmed its results using multispectral analysis. The triple-stain method was significantly more sensitive in detecting invasive events than the standard H&E staining method and easily discriminated between blood and lymphatic vessel invasion. Invasive events were present in blood and/or lymphatic vessels in the majority of patients with and without presentation of lymph node metastasis, indicating that vessel invasion in this cancer model is common and is not a rate-limiting step for lymph node metastasis.

Key Words: vessel invasion, metastasis, oral squamous cell carcinoma, immunohistochemistry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2008

 
Purchase HCS Short Course Manual on HCS site