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JHC exPRESS: First Published September 15, 2008. doi:10.1369/jhc.2008.951582
Journal of Histochemistry and Cytochemistry
Copyright © 2008 Di Giusto et al.

A more recent version of this article appeared on January 1, 2009.
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Articles

Oat5 and NaDC1 Protein Abundances in Kidney and Urine Following Renal Ischemic Reperfusion Injury

Gisela Di Giusto 1, Naohiko Anzai 1, Hitoshi Endou 1 and Adriana M. Torres 1*

1 Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, CONICET, Rosario, Argentina (GDG,AMT), and Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan (NA,HE)

* To whom correspondence should be addressed. E-mail: admotorres{at}yahoo.com.ar.

Submitted on April 3, 2008
Accepted on 29 August 2008


  Abstract
The aim of this study was to evaluate the abundances of the organic anion transporter 5 (Oat5) and the sodium-dicarboxylate cotransporter 1 (NaDC1) in kidney and urine following renal ischemic reperfusion injury. Renal injury was induced in male Wistar rats by occlusion of both renal pedicles for 0 (Group Sham), 5 (Group I5R60), or 60 (Group I60R60) minutes. The studies were performed after 60 minutes of reperfusion. The expression of Oat5 and NaDC1 was evaluated by immunohistochemistry and Western blotting. Oat5 and NaDC1 abundances and alkaline phosphatase activity (AP) were assayed in urine. A decrease expression in renal homogenates and apical membranes and an increase urinary excretion of Oat5 and NaDC1 were observed in I60R60 rats as well as alterations of other widely used parameters for renal dysfunction and injury (plasma creatinine, urinary AP activity, kidney weight, histological lesions). By contrast, in I5R60 group only an increase of urinary excretion of Oat5 and mild histopathological damage was detected. The present is the first report for Oat5 and NaDC1 detection in urine. These results suggest that urinary excretion of Oat5 might be an early indicator of renal dysfunction useful for detection of even minor alterations in renal structural and functional integrity.

Key Words: acute renal failure, ischemia and reperfusion, Oat5, NaDC1


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