Secreted Protein Acidic and Rich in Cysteine (SPARC) in Human Skeletal Muscle

Louise H. Jørgensen ¹, Stine J. Petersson ¹, Jeeva Sellathurai ¹, Ditte C. Andersen ¹, Susanne Thayssen ¹, Dorte J. Sant ¹, Charlotte H. Jensen ¹ and Henrik D. Schrøder ^1*

¹ Institute of Clinical Research (LHJ,SJP,JS,HDS) and Immunology and Microbiology, Institute of Medical Biology (DCA,CHJ), University of Southern Denmark, Odense, Denmark, and Department of Clinical Pathology, Odense University Hospital, Odense, Denmark (LHJ,SJP,JS,DCA,ST,DJS,HDS)

^* To whom correspondence should be addressed. E-mail: henrik.daa.schroeder{at}ouh.regionsyddanmark.dk.

Submitted on May 21, 2008
Accepted on 3 September 2008

	Abstract

Secreted Protein Acidic and Rich in Cysteine (SPARC)/osteonectin is expressed in different tissues during remodelling and repair suggesting a function in regeneration. Several gene expression studies indicated that SPARC was expressed in response to muscle damage. Studies on myoblasts further indicated a function of SPARC in skeletal muscle. We therefore found it of interest to investigate SPARC expression in human skeletal muscle during development and in biopsies from duchenne and becker muscular dystrophy and congenital muscular dystrophy, congenital myopathy, inclusion body myositis and polymyositis patients to analyze SPARC expression in a selected range of inherited and idiopathic muscle wasting diseases. SPARC+ cells were observed both in fetal and neonatal muscle, and in addition fetal myofibers were observed to express SPARC at the age of 15-16 weeks. SPARC protein was detected in the majority of analyzed muscle biopsies (23 of 24), mainly in mononuclear cells of which few were pax7-positive. Myotubes and regenerating myofibers also expressed SPARC. The expression-degree appeared to reflect the severity of the lesion. In accordance with these in vivo findings primary human-derived satellite cells were found to express SPARC both during proliferation and differentiation in vitro. In conclusion, this study shows SPARC expression both during muscle development and in regenerating muscle. The expression is detected both in satellite cells/myoblasts and in myotubes and muscle fibers, indicating a role for SPARC in the skeletal muscle compartment.

Key Words: skeletal muscle, SPARC/osteonectin/BM-40, congenital myopathy, muscular dystrophy, myogenesis, satellite cell

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