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JHC exPRESS: First Published September 29, 2008. doi:10.1369/jhc.2008.951996
Journal of Histochemistry and Cytochemistry
Copyright © 2008 Lobo et al.

A more recent version of this article appeared on January 1, 2009.
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Articles

Hormone-sensitive Lipase Expression and Immunohistochemical Localization in the Rat Ovary, Oviduct, and Uterus

María V.T. Lobo 1, Lydia Huerta 1, María Isabel Arenas 1, Rebeca Busto 1, Miguel Angel Lasunción 1 and Antonia Martín-Hidalgo 1*

1 Department of Cell Biology and Genetics, Alcalá de Henares University, Madrid, Spain (MVTL,MIA); Service of Biochemistry-Research Department, Ramón y Cajal Hospital, Madrid, Spain (LH,RB,MAL,AM-H); and Ciber Fisiopatología Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain (LH,RB,MAL,AM-H)

* To whom correspondence should be addressed. E-mail: antonia.martin{at}hrc.es.

Submitted on May 28, 2008
Accepted on 12 September 2008


  Abstract
Hormone-sensitive lipase (HSL) is a key regulator of cholesterol esters metabolism .The aim of this study was to determine HSL localization in rat female reproductive organs during the ovarian cycle by immunohistochemical methods. HSL was located in the ovarian epithelium. The granulosa cells and oocytes of primordial follicles were immunonegative. In mature follicles, HSL was found in oocytes, theca and granulosa cells. However, HSL expression in theca cells and oocytes decreased during follicular atresia. Luteal cells showed HSL staining in cytoplasm during proestrus and estrus, in nucleus during metestrus, and cytoplasm and nucleus during diestrus. In the tubaric ampulla, HSL was located in the epithelial cells nuclei and in the cilia during proestrus and estrus, but mainly in nucleus during metestrus and diestrus. In the isthmus, cells showed HSL immunolabelling in the nucleus and cilia during proestrus, but only in the cilia during estrus, metestrus and diestrus. In the uterus, HSL was found in the epithelial cells nuclei. HSL-immunoreactive bands at 84, 67, 54 and 43 kDa were found in rat female reproductive organs. HSL labelling in the nucleus of epithelial and germ cells suggests an, as yet, unknown function for this protein, probably related to oogenesis and cell proliferation.

Key Words: hormone-sensitive lipase, ovary, oviduct, uterus, oogenesis, immunohistochemistry, cholesterol esters


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