Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

JHC exPRESS: First Published October 27, 2008. doi:10.1369/jhc.2008.952531
Journal of Histochemistry and Cytochemistry
Copyright © 2008 Macgregor et al.

A more recent version of this article appeared on March 1, 2009.
This Article
Right arrow exPRESS PDF
Right arrow All Versions of this Article:
jhc.2008.952531v1
57/3/207    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Macgregor, A. M.
Right arrow Articles by Halushka, M. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Macgregor, A. M.
Right arrow Articles by Halushka, M. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Articles

Tissue Inhibitor of Matrix Metalloproteinase-3 Levels in the Extracellular Matrix of Lung, Kidney, and Eye Increase With Age

Anne M. Macgregor 1, Charles G. Eberhart 1, Mostafa Fraig 1, Jie Lu 1 and Marc K. Halushka 1*

1 Department of Pathology (AMM,CGE,MF,JL,MKH) and Department of Ophthalmology (CGE), Johns Hopkins University School of Medicine, Baltimore, Maryland

* To whom correspondence should be addressed. E-mail: mhalush1{at}jhmi.edu.

Submitted on August 19, 2008
Accepted on 16 October 2008


  Abstract
Tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) is an important regulator of matrix metalloproteinase activity in many types of disease, including atherosclerosis, neoplasia and inflammatory conditions. Among TIMPs, TIMP-3 uniquely binds the extracellular matrix (ECM). We performed immunohistochemical staining on seventeen tissue microarrays (TMAs) containing over 1,500 samples to determine the location of ECM TIMP-3 staining in a variety of predominantly vascular tissues. We found a unique pattern of TIMP-3 staining in the ECM of renal arterioles, small pulmonary vessels and parenchyma, and in Bruch’s membrane in the retina. There was no staining in larger caliber arteries including coronary and internal mammary arteries. TIMP-3 protein accumulation was found to be an age-dependent phenomenon, with staining appearing in all three tissues in early adulthood and becoming more robust among the elderly. These findings may help to explain the late onset of the TIMP-3 associated ocular diseases Sorsby fundus dystrophy and age-related macular degeneration, and suggest a similar phenomenon could be at work in other age-related conditions.

Key Words: TIMP-3, extracellular matrix, immunohistochemistry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
T. C. Cornish, S. M. Bagnasco, A. M. Macgregor, J. Lu, E. Selvin, and M. K. Halushka
Glomerular Protein Levels of Matrix Metalloproteinase-1 and Tissue Inhibitor of Metalloproteinase-1 Are Lower in Diabetic Subjects
J. Histochem. Cytochem., November 1, 2009; 57(11): 995 - 1001.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2008

 
Purchase HCS Short Course Manual on HCS site