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JHC exPRESS: First Published October 14, 2008. doi:10.1369/jhc.2008.952770
Journal of Histochemistry and Cytochemistry
Copyright © 2008 Saper

A more recent version of this article appeared on January 1, 2009.
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Perspectives

A Guide to the Perplexed on the Specificity of Antibodies

Clifford B. Saper 1*

1 Department of Neurology and Program in Neuroscience, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, Massachusetts

* To whom correspondence should be addressed. E-mail: csaper{at}bidmc.harvard.edu.

Submitted on September 16, 2008
Accepted on 16 September 2008


  Abstract
Many investigators are unaware of the potential problems with specificity of antibodies, and the need to document antibody characterization meticulously for each antibody that is used. In this review, we consider the principles of antibody action, and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation.
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