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JHC exPRESS: First Published December 24, 2008. doi:10.1369/jhc.2008.952895
Journal of Histochemistry and Cytochemistry
Copyright © 2008 Odaka

A more recent version of this article appeared on April 1, 2009.
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Localization of Mesenchymal Cells in Adult Mouse Thymus: Their Abnormal Distribution in Mice With Disorganization of Thymic Medullary Epithelium

Chikako Odaka 1*

1 Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, Japan

* To whom correspondence should be addressed. E-mail: odaka{at}nih.go.jp.

Submitted on September 24, 2008
Accepted on 4 December 2008


  Abstract
Thymic mesenchymal cells are known to be important for early fetal thymus development into a functionally mature organ supporting T cell differentiation. We examined the expression of mesenchymal markers: pan-mesenchymal marker ER-TR7, desmin, {alpha}-smooth muscle actin ({alpha}-SMA), and {alpha}- and {beta}-chain of platelet-derived growth factor receptor (PDGFR{alpha}, PDGFR{beta}), in thymi of normal adult mice. Desmin and ER-TR7 revealed specific staining in capsule, septa, and perivascular cells. Most perivascular cells highly expressed PDGFR{beta} at the same levels as desmin. Low expression of PDGFR{alpha} was detected in capsule, intralobular septa, and some perivascular cells of normal adult thymi. {alpha}-SMA, used to identify vascular smooth muscle cells (VSMCs), was detectable on arterioles and some large venules but not on capillaries. Thus, desmin, PDGFR{alpha}, and PDGFR{beta} were localized in the capsule, septa, and perivascular cells in thymus of adult mouse, though there are differences in the expression level among these markers. On the other hand, the expression of mesenchymal markers was detectable in the region of thymic medullary epithelium of lymphotoxin beta receptor (LT{beta}R)-deficient mice and plt/plt mice, indicating that mesenchymal cells were abnormally localized in the region. These results suggest that disorganization of medullary epithelium may accompany with aberrant distribution of mesenchyme in adult mouse thymus.

Key Words: thymus mesenchymal cells, lymphotoxin beta receptor-deficient mice, Plt/plt mice, adult


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