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JHC exPRESS: First Published March 2, 2009. doi:10.1369/jhc.2009.953059
Journal of Histochemistry and Cytochemistry
Copyright © 2009 Gomez-Sanchez et al.

A more recent version of this article appeared on July 1, 2009.
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Immunohistochemical Demonstration of the Mineralocorticoid Receptor, 11{beta}-Hydroxysteroid Dehydrogenase 1 and 2, and Hexose-6-Phosphate Dehydrogenase in Rat Ovary

Elise P. Gomez-Sanchez 1*, Miriam T. Gomez-Sanchez 1, Angela F. de Rodriguez 1, Damian G. Romero 1, Mary P. Warden 1, Maria W. Plonczynski 1 and Celso E. Gomez-Sanchez 1

1 Research Service, G.V. (Sonny) Montgomery VA Medical Center, Department of Medicine, Division of Endocrinology, The University of Mississippi Medical Center, Jackson, Mississippi

* To whom correspondence should be addressed. E-mail: egomez-sanchez{at}medicine.umsmed.edu.

Submitted on October 15, 2008
Accepted on 5 February 2009


  Abstract
An immunohistochemical (IHC) survey using several monoclonal antibodies against different portions of the rat mineralocorticoid receptor (MR) molecule demonstrated significant specific MR immunoreactivity in the ovary, prompting further study of the localization of MR and determinants of extrinsic MR ligand specificity, 11ß-hydroxysteroid dehydrogenase 1 and 2 (11ß-HSD), and hexose-6-phosphate dehydrogenase (H6PDH). MR expression (real-time RT-PCR and western blot) did not differ significantly in whole rat ovaries at early diestrus, late diestrus, estrus, and a few hours after ovulation. MR immunostaining was most intense in corporal lutea cells, light to moderate in oocytes and granulosa cells, and least intense in theca cells. Light immunoreactivity for 11ß-HSD 2 occurred in most cells with some mural granulosa cells of mature follicles staining more strongly. The distribution of immunoreactivity for 11ß-HSD 1 and H6PDH required to generate NADPH, the co-factor required for reductase activity of 11ß-HSD 1, was similar, with the most intense staining in the cytoplasm of corporal lutea and theca cells and light or no staining in the granulosa and oocytes. MR function in the ovary is yet unclear, but distinct patterns of distribution of the 11ß-HSD 2, 11ß-HSD1 and H6PDH suggest that the ligand for MR activation in different cells of the ovary may be differentially regulated.

Key Words: corticosterone, cortisol, aldosterone


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Home page
 Exp PhysiolHome page
E. P. Gomez-Sanchez, C. M. Gomez-Sanchez, M. Plonczynski, and C. E. Gomez-Sanchez
Aldosterone synthesis in the brain contributes to Dahl salt-sensitive rat hypertension
Exp Physiol, January 1, 2010; 95(1): 120 - 130.
[Abstract] [Full Text] [PDF]



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