Probasin Promoter Driven Expression of ID1 Is not Sufficient for Carcinogenesis in Rodent Prostate

Robert Salomon ¹, Lei Young ¹, Duncan MacLeod ¹, Xiao-Ling Yu ¹ and Qihan Dong ^1*

¹ Central Clinical School, The University of Sydney, Department of Endocrinology, and Sydney Cancer Centre (RS,LY,X-LY,QD) and Department of Anatomical Pathology (DM), Royal Prince Alfred Hospital, Sydney, Australia

^* To whom correspondence should be addressed. E-mail: qhd{at}med.usyd.edu.au.

Submitted on November 6, 2008
Accepted on 11 February 2009

	Abstract

Inhibitor of DNA-binding-1 (ID1) negatively regulates cell differentiation and senescence, and enhances cellular proliferation and angiogenesis. Elevated levels of ID1 have been found in a variety of cancer including prostate but whether ID1 has a tumourigenic role remains to be established. We established heterozygous and homozygous ID1 transgenic mouse lines driven by prostate specific probasin promoter (-426 to +28 bp). Although elevated levels of ID1 were confirmed by RT-PCR, immunohistochemistry and Western blot analysis, there were no morphological changes identified in the prostate of transgenic mice at 26 and 52 weeks. Thus, overexpression of ID1 alone is not sufficient to drive neoplastic change in mouse prostate.

Key Words: inhibitor of DNA-binding-1, probasin, prostate, cancer, transgenic

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2009