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JHC exPRESS: First Published May 6, 2005. doi:10.1369/jhc.4A6548.2005
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A more recent version of this article appeared on August 1, 2005.
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ARTICLE

Parathyroid Hormone-related Peptide (hPTHrP) and Parathyroid Hormone-related-peptide Receptor Type 1 (PTHR1) Expression in Human Thymus

Marco Gessi 1, Giovanni Monego 1, Libero Lauriola 1, Nicola Maggiano 1 and Franco O. Ranelletti 1*

1 Department of Pathology (MG,LL,NM), Department of Anatomy and Cellular Biology (GM), and Department of Histology (FOR), Catholic University, Rome, Italy

* To whom correspondence should be addressed. E-mail: E-mail: ranelletti{at}rm.unicatt.it.

Submitted on October 13, 2004
Accepted on 22 February 2005


  Abstract
Parathyroid hormone-related peptide (hPTHrP) is expressed in human tissues and regulates cellular proliferation, differentiation and apoptosis, by an autocrine/paracrine loop. In rodent thymus, both parathormone and PTHrP are expressed by thymic epithelial cells (TEC). The present study demonstrated by RT-PCR and immunohistochemistry that hPTHrP and PTHR1 were expressed in human thymus at both RNA and protein levels. hPTHrP was expressed mainly in the thymic medulla by epithelial (cytokeratin positive), mature dendritic (CD 40+/86+) and plasmacytoid (IL-3R{alpha} +) cells. This protein was also present in some cells forming Hassall's bodies and few subcapsular and cortical TEC. PTHR1 was expressed by scattered subcapsular and cortical TEC and by rare TEC in the medulla. Thymocytes did not express either hPTHrP or PTHR1. Primary cultures of human TEC revealed the presence of both hPTHrP and PTHR1 mRNAs, confirming the capacity of TEC to sinthesize both peptides. Moreover, synthetic (1-39) hPTHrP peptide administered on cultured TEC induced the expression of IL-6 mRNA suggesting that hPTHrP can regulate thymic functions by inducing in TEC the expression of IL-6 which is involved in the development and maturation of thymocytes.

Key Words: human thymus, hPTHrP, PTHR1, thymic epithelial cells, dendritic cells


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