Localization of P2X and P2Y Receptors in Dorsal Root Ganglia of the Cat

Huai-Zhen Ruan ¹, Lori A. Birder ¹, William C. de Groat ¹, Changfeng Tai ¹, James Roppolo ¹, Charles A. Buffington ¹ and Geoffrey Burnstock ^1*

¹ Autonomic Neuroscience Centre, Royal Free & University College Medical School, London, United Kingdom (H-ZR,GB); Department of Neurobiology, Third Military Medical University, Chongqing, China (H-ZR); University of Pittsburgh School of Medicine, Departments of Medicine (LAB) and Pharmacology (LAB,WCdG,CT,JR), Pittsburgh, Pennsylvania; and Ohio State University, Department of Clinical Veterinary Sciences, Columbus, Ohio (CAB)

^* To whom correspondence should be addressed. E-mail: g.burnstock{at}ucl.ac.uk.

Submitted on October 18, 2004
Accepted on 4 May 2005

	Abstract

The distribution of P2x and P2Y receptor subtypes in upper lumbosacral cat dorsal root ganglia (DRG) has been investigated using immunohistochemistry. Intensity of immunoreactivity for six P2x receptors (P2x₅ receptors were immuno-negative) and the three P2Y receptors examined in cat DRG was in the order of P2Y₂=P2Y₄>P2x₃>P2x₂=P2x₇>P2x₆>P2x₁=P2x₄>P2Y₁. P2x₃, P2Y₂ and P2Y₄ receptor polyclonal antibodies stained 33.8%, 35.3% and 47.6% of DRG neurons, respectively. Most P2Y_2, P2x_1, P2x_3, P2x₄ and P2x₆ receptor staining was detected in small- and medium-diameter neurons. However, P2Y_4, P2x₂ and P2x₇ staining was present in large- as well as small-diameter neurons. Double-labeling immunohistochemistry showed that 90.8%, 32.1% and 2.4% of P2x₃ receptor-positive neurons coexpressed IB₄, CGRP and NF200, respectively; while 67.4%, 41.3% and 39.1% of P2Y₄ receptor-positive neurones coexpressed IB₄, CGRP and NF200, respectively. 18.8%, 16.6% and 63.5% of P2Y₂ receptor-positive neurons also stained for IB₄, CGRP and NF200, respectively. Only 30% of DRG neurones in cat were P2x₃ immunoreactive compared with 90% in rat and in mouse. A further difference was the low expression of P2Y₁ receptors in cat DRG neurones compared with over 80% of the neurons in rat. Many small-diameter neurones were NF200-positive in cat, again differing from rat and mouse.

Key Words: Purinoceptors, P2X receptor, P2Y receptors, ATP, dorsal root ganglion, cat

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