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JHC exPRESS: First Published May 6, 2005. doi:10.1369/jhc.4A6568.2005
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A more recent version of this article appeared on August 1, 2005.
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ARTICLE

Profiles of PrKX Expression in Developmental Mouse Embryo and Human Tissues

Wei Li 1, Zu-Xi Yu 1 and Robert M. Kotin 1*

1 Laboratory of Biochemical Genetics and Pathology Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland

* To whom correspondence should be addressed. E-mail: E-mail: kotinr{at}nhlbi.nih.gov.

Submitted on November 3, 2004
Accepted on 17 March 2005


  Abstract
Protein kinase X (PrKX), karyotypically located on the human X chromosome, is a type I cAMP-dependent protein kinase. Although a specific role for PrKX has not yet been defined, PrKX gene expression in mouse and human tissues has been profiled only by in situ hybridization and northern blot analyses and not by protein expression. To determine the PrKX protein levels more precisely, we developed specific anti-PrKX antibodies and examined gestational staged mouse embryo sections by immunohistochemistry. These results showed that PrKX is ubiquitously distributed and highly expressed in murine central nervous system and heart tissues in early developmental stages and in most organs at later stages, but was not detected in either connective tissues or bone. Using western blots to detect PrKX, total protein extracts from eight different adult or fetal human tissues including brain, heart, kidney, liver, lung, pancreas, spleen and thymus were analyzed. Although PrKX protein was present in each of the tissues tested, the protein levels varied depending on tissue type and development stage. Very low protein levels were found in heart tissues from a five month old fetus and adult, whereas PrKX proteins were more abundant in fetal brain, kidney and liver tissues compared with adult samples of the same tissue type.

Key Words: PrKX, type I cAMP-dependent protein kinase, immunohistological staining, western blot


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