Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
JHC exPRESS: First Published May 27, 2005. doi:10.1369/jhc.4A6597.2005
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on October 1, 2005.
This Article
Right arrow exPRESS PDF
Right arrow All Versions of this Article:
jhc.4A6597.2005v1
53/10/1293    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Määttä, M.
Right arrow Articles by Virtanen, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Määttä, M.
Right arrow Articles by Virtanen, I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Articles

Differential Expression of Laminin Isoforms in Ovarian Epithelial Carcinomas Suggesting Different Origin and Providing Tools for Differential Diagnosis

Marko Määttä 1*, Ralf Bützow 1, Jani Luostarinen 1, Noora Petäjäniemi 1, Taina Pihlajaniemi 1, Sirpa Salo 1, Kaoru Miyazaki 1, Helena Autio-Harmainen 1 and Ismo Virtanen 1

1 Department of Pathology, University of Oulu, Oulu, Finland (MM,HA-H); Department of Pathology, HUSLAB, Haartman Institute and Department of Obstetrics and Gynecology, Research Laboratory, Biomedicum, Helsinki University Central Hospital, Finland (RB); Collagen Research Unit, Biocenter Oulu, Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland (JL,TP); Institute of Biomedicine/Anatomy, University of Helsinki, Helsinki, Finland (NP,IV); Department of Biochemistry, University of Oulu, Oulu, Finland (SS); and Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Maioka-cho, Totsuka-ku, Yokohama, Japan (KM)

* To whom correspondence should be addressed. E-mail: mmaatta{at}mailcity.com.

Submitted on December 11, 2004
Accepted on 19 April 2005


  Abstract
Immunohistochemistry was used to study the distribution of laminin (Ln) chains, collagen types IV ({alpha} 1/2), VII and XVIII and Lutheran antigen (Lu) in 36 frozen ovarian carcinoma samples. Surface epithelial BM of the normal ovary showed immunoreactivity for Ln {alpha}1, {alpha}3-{alpha}5, {beta}1-3, {gamma}1 and {gamma}2 chains and type IV and XVIII collagens. Chains of Ln-5 ({alpha}3{beta}3{gamma}2) and Ln-10 ({alpha}5{beta}1{gamma}1) as well as type IV and XVIII collagens were found in most tumor BMs, but Ln {alpha}2 chain and type VII collagen were detected only in few tumors. Contrary to serous tumors BMs of mucinous carcinomas showed Ln {alpha}4 chain, but not Ln {alpha}1 and {beta}2 chains. Ln {alpha}1 chain was found in most endometrioid carcinomas, while chains of Ln-5 were only moderately detectable in comparison with serous and mucinous carcinomas. In the normal ovary Lu immunoreactivity was confined to basal aspect in the ovarian epithelial cells, but in tumor specimens Lu immunostainings showed variable polarized and nonpolarized patterns. The results suggest that the three types of ovarian carcinoma have distinct differences in their Ln distribution and can be grouped based on their expression pattern. This suggests that they may have histogenetically different precursors and may help to distinguish these tumors from each other.

Key Words: basement membrane, carcinoma, collagen, invasion, laminin, lutheran, ovary


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2005