Development of In Situ Zymography to Localize Active Matrix Metalloproteinase-7 (Matrilysin-1)

Ryoichi Nemori ¹, Masayoshi Yamamoto ¹, Fumio Kataoka ¹, Gakuji Hashimoto ¹, Hiroshi Arakatsu ¹, Takayuki Shiomi ¹ and Yasunori Okada ^1*

¹ Advanced Core Technology Laboratories, Fuji Photo Film Co., Ltd., Kanagawa, Japan (RN,MY,HA) and Department of Pathology, School of Medicine, Keio University, Tokyo, Japan (FK,GH,TS,YO)

^* To whom correspondence should be addressed. E-mail: okada{at}sc.itc.keio.ac.jp.

Submitted on January 23, 2005
Accepted on 11 May 2005

	Abstract

Matrix metalloproteinase-7 (MMP-7) is up-regulated during carcinogenesis and its expression correlates with metastasis of human endometrial and gastrointestinal carcinomas. In the present study, we have developed a new method to localize the activity of MMP-7 within tissues. Polyethylene terephthalate films were uniformly coated with cross-linked carboxymethylated transferrin (CCm-Tf) as a substrate and incubated with frozen tissue sections mounted on the films. CCm-Tf on the films was degraded selectively by MMP-7, but showed little or no susceptibility to MMP-1, 2, 3, 9, 13, MT1-MMP, MT3-MMP or ADAMTS4. Although some serine proteinases such as elastase also digested CCm-Tf, CCm-Tf films impregnated with serine proteinase inhibitors prevented the digestion. When frozen sections of human endometrial carcinoma and lung carcinoma tissues were incubated on CCm-Tf films or those treated with proteinase inhibitors, the activity was detected in the carcinoma cell nests, where MMP-7 was immunolocalized. The present in situ zymography using CCm-Tf may be a useful method to analyze the functions of MMP-7 in pathophysiological conditions.

Key Words: matrix metalloproteinase-7, matrilysin-1, in situ zymography, proteolytic activity, tissue localization, carcinoma invasion

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