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JHC exPRESS: First Published April 3, 2006. doi:10.1369/jhc.5A6733.2006
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on September 1, 2006.
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Immunohistochemical Study of Cell Proliferation, Bcl-2, p53 and Caspase-3 Expression on Preneoplastic Changes Induced by Cadmium and Zinc Chloride in the Ventral Rat Prostate

Riánsares Arriazu 1, José M. Pozuelo 1, Nuno Henriques-Gil 1, Teresa Perucho 1, Rocío Martín 1, Rosario Rodríguez 1 and Luis Santamaría 1*

1 Cell Biology and Histology Laboratory (RA,JMP,RR) and Genetics Laboratory (NH-G,TP), San Pablo-CEU University, Madrid, Spain; Service of Pathology, Hospital N. Sra. de Sonsoles, Ávila, Spain (RM); and Department of Morphology, School of Medicine, Autonomous University of Madrid, Madrid, Spain (LS)

* To whom correspondence should be addressed. E-mail: luis.santamaria{at}uam.es.

Submitted on May 17, 2005
Accepted on 11 March 2006


  Abstract
This work was directed to evaluate the immunoexpression of markers for apoptosis, resistance to apoptosis, and cell proliferation; and estimates of nuclear size, in ventral prostate of rats treated with cadmium chloride (Cd) and cadmium plus zinc chloride (Cd+Zn), because a possible protective effect of the zinc has been postulated. The following variables were studied: volume fraction (VF) of Bcl-2 immunostaining, percentage of cells immunoreactive to PCNA (LIPCNA) and p53 (LIp53), numerical density of caspase-3 immunoreactive cells (NV caspase-3), estimates of volume-weighted mean nuclear volume ({upsilon}V). The LIPCNA and VF of Bcl-2 were significantly increased in the treated animals. The dysplasias (independently of their origin) showed a significant increase of the LIp53, NV caspase-3, and {upsilon}V in comparison with normal acini from treated and control animals. It can be concluded that cell proliferation is enhanced in long time cadmium expossed rats, the exposure to zinc combined with cadmium had no effect over any of the variables studied when comparing with normal acini, and that the increase of nuclear {upsilon}V could announce a more agressive behavior for pretumoral lesions.

Key Words: rat prostate, cadmium, zinc, dysplasia, immunohistochemistry


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