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JHC exPRESS: First Published December 12, 2005. doi:10.1369/jhc.5A6755.2005
Journal of Histochemistry and Cytochemistry
Copyright © 2005 Yamamoto & Taniguchi


A more recent version of this article appeared on April 1, 2006.
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Expression of Tandem P Domain K+ Channel, TREK-1, in the Rat Carotid Body

Y. Yamamoto 1* and K. Taniguchi 1

1 Laboratory of Veterinary Anatomy, Faculty of Agriculture, Iwate University, Morioka, Japan

* To whom correspondence should be addressed. E-mail: yyoshio{at}iwate-u.ac.jp.

Submitted on June 13, 2005
Accepted on 22 November 2005


   Abstract
TREK-1 is one of the important potassium channels to regulate membrane excitability. To examine the distribution of TREK-1 in the rat carotid body, we performed RT-PCR for mRNA expression and in situ hybridization and immunohistochemistry for tissue distribution of TREK-1. RT-PCR detected mRNA expression of TREK-1 in the carotid body. Furthermore, in situ hybridization revealed the localization of TREK-1 mRNA in the glomus cells. TREK-1 immunoreactivity was mainly distributed in the glomus cells and nerve fibers in the carotid body. TREK-1 may modulate potassium current of glomus cells and/or afferent nerve endings in the rat carotid body.

Key Words: 2P-domain potassium channel, carotid body, membrane potential, immunocytochemistry, rat


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