Naturally Occurring Anti Albumin Antibodies Are Responsible for False Positivity in Diagnosis of Autoimmune Premature Ovarian Failure

Eusebio S. Pires ¹, Priyanka P. Parte ¹, Perveen K. Meherji ¹, Shagufta A. Khan ¹ and Vrinda V. Khole ^1*

¹ Department of Gamete Immuno Biology (ESP,PPP,SAK,VVK) and Department of Endocrinology and Infertility (PKM), National Institute for Research in Reproductive Health, Parel, India

^* To whom correspondence should be addressed. E-mail: kholevv{at}icmr.org.in.

Submitted on July 11, 2005
Accepted on 27 October 2005

	Abstract

Autoimmunity is a well-established causative factor of premature ovarian failure (POF) and evidence for the same is well reported in literature. Detection of specific autoantibodies remains the most practical clinical research marker of any autoimmune disease. Variation in efficiency and specificity in the detection of ovarian autoantibodies has been reported. However, the frequency of false positivity and a solution to overcome this has not been reported. Herein, we report autoantibody to albumin as the likely responsible agent for the false positivity. Our data indicates that presence of naturally existing auto albumin antibodies in circulation of normal women is responsible for the false signal seen in SDS-PAGE Western blot analysis and in immunohistochemistry (IHC). Having shown the presence of anti-albumin antibody in normal women as well as in POF patient’s sera, we have developed a novel-blocking agent to overcome this problem. A high titer polyclonal antibody against human serum albumin was generated. This antibody showed immunoreactivity to albumin obtained from various sources. Preincubation of Western blots and IHC sections with this antibody drastically reduced background signals. The advantage of using this blocking was evident by identification of specific anti ovarian antibodies in a group of POF patients. This blocking procedure made it possible to get a clear indication of the ovarian antibody status in women presenting with autoimmune POF (AI-POF).

Key Words: autoimmune POF, false positives, blockings, autoantibodies, anti albumin antibodies

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