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JHC exPRESS: First Published March 3, 2006. doi:10.1369/jhc.5A6792.2006
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A more recent version of this article appeared on August 1, 2006.
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The Secretory Granule Protein Syncollin Localizes to HL-60 Cells and Neutrophils

Jan-Philipp Bach 1, Heike Borta 1, Waltraud Ackermann 1, Floriane Faust 1, Oliver Borchers 1 and Michael Schrader 1*

1 Department of Cell Biology and Cell Pathology, University of Marburg, Marburg, Germany

* To whom correspondence should be addressed. E-mail: schrader{at}mailer.uni-marburg.de.

Submitted on July 26, 2005
Accepted on 22 February 2006


  Abstract
The secretory granule protein syncollin has first been identified in the exocrine pancreas, where a population of the protein is associated with the luminal surface of the zymogen granule membrane. In this study, we provide first morphological and biochemical evidence that in addition to its pancreatic localization syncollin is also present in neutrophilic granulocytes of rat and human origin. By immunohistological studies, syncollin was detected in neutrophilic granulocytes of the spleen. Furthermore, syncollin is expressed by the promyelocytic HL-60 cells, where it is stored in azurophilic granules and a vesicular compartment. These findings were confirmed by fractionation experiments and immunoelectron microscopy. Treatment with a phorbol ester triggered the release of syncollin indicating that in HL-60 cells it is a secretory protein which can be mobilized upon stimulation. A putative role for syncollin in host defense is discussed.

Key Words: pancreas, granule biogenesis, neutrophil, secretion, HL-60, antimicrobial


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