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JHC exPRESS: First Published March 3, 2006. doi:10.1369/jhc.5A6877.2006
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A more recent version of this article appeared on July 1, 2006.
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Internalization and Transcytosis of Pancreatic Enzymes by the Intestinal Mucosa

Maryse Cloutier 1, Diane Gingras 1 and Moïse Bendayan 1*

1 Department of Pathology and Cell Biology, University of Montreal, Montreal, Quebec, Canada

* To whom correspondence should be addressed. E-mail: Moise.Bendayan{at}umontreal.ca.

Submitted on November 11, 2005
Accepted on 17 February 2006


  Abstract
As early as the beginning of the 20th century some data indicated that macromolecules can cross the intestinal mucosa to reach the blood. Further evidence was added along the years, however, pathways for this transport still remain to be established. We herein report the transfer of two pancreatic enzymes, amylase and lipase, from the intestinal lumen to the blood. Both are present in higher concentrations in the intestinal mucosa and in blood of fed rats. Upon cholinergic stimulation of pancreatic secretion, not only enzymes concentrations in blood rose but evidence for internalization by duodenal enterocytes was obtained. Following insertion of fluorochrome-tagged amylase and lipase into the duodenal lumen of fasting rats, blood and intestinal tissues were sampled at different time points. Serum activities for both enzymes clearly increased with time. Light microscopy established internalization of both proteins by duodenal enterocytes and immunogold outlined the pathway taken by both proteins across the enterocytes. From the intestinal lumen, the enzymes are channelled through the endosomal compartment to the Golgi apparatus and to the basolateral membrane reaching the interstitial space and blood circulation. Thereby, transcytosis through the intestinal mucosa represents an access route for pancreatic enzymes to reach blood circulation.

Key Words: amylase, lipase, duodenal wall, pancreas, transcytosis, immunocytochemistry


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