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JHC exPRESS: First Published January 23, 2006. doi:10.1369/jhc.5A6889.2006
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on June 1, 2006.
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Articles

Laminin-311 (Laminin-6) Fiber Assembly by Type I-like Alveolar Cells

Phillip J. DeBiase 1, Kimberly Lane 1, Scott Budinger 1, Karen Ridge 1, Mindy Wilson 1 and Jonathan C.R. Jones 1*

1 Division of Pulmonary Medicine and the Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois

* To whom correspondence should be addressed. E-mail: j-jones3{at}northwestern.edu.

Submitted on November 11, 2005
Accepted on 5 January 2006


  Abstract
Two epithelial cell types cover the alveolar surface of the lung. Type II alveolar epithelial cells produce surfactant and, during development or following wounding, give rise to type I cells which are involved in gas exchange and alveolar fluid homeostasis. In culture, freshly isolated alveolar type II cells assume a more squamous (type I-like) appearance within 4 days after plating. They assemble numerous focal adhesions that associate with the actin cytoskeleton at the cell margins. These alveolar epithelial cells lose expression of type II cell markers, including SP-C, and, by 4 days in culture, express the type I cell marker T1 alpha. Those cells that express T1 alpha also deposit fibers of laminin-311 in their matrix. The latter appears related to their development of a type I phenotype since freshly isolated, primary type I cells also assemble laminin-311-rich fibers in vitro. A {beta}1 integrin antibody antagonist inhibits the assembly of laminin-311 matrix fibers by alveolar cells. Moreover the formation of laminin fibers is dependent on the activity of the small GTPases and is perturbed by ML-7, a myosin light chain kinase inhibitor. In summary, our data indicate that assembly of laminin-311 fibers by lung epithelial cells is integrin- and actin cytoskeleton-dependent and that these fibers are characteristic of type I alveolar cells.

Key Words: matrix adhesion, cytoskeleton, differentiation


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