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JHC exPRESS: First Published May 30, 2006. doi:10.1369/jhc.5A6892.2006
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A more recent version of this article appeared on October 1, 2006.
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PET-blot Analysis Contributes to BSE Strain Recognition in C57Bl/6 Mice

Stéphane Lezmi 1, Anna Bencsik 1 and Thierry Baron 1*

1 Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité ATNC, Lyon, France

* To whom correspondence should be addressed. E-mail: t.baron{at}lyon.afssa.fr.

Submitted on November 24, 2005
Accepted on 16 May 2006


  Abstract
Identification of the strain of agent responsible for the bovine spongiform encephalopathy (BSE) can be made on histological basis, through the analysis of both distribution and intensity of brain vacuolar lesions after BSE transmission to mouse. Another useful way to distinguish the BSE agent from other prion strains is the study of the abnormal prion protein (PrPres) distribution. For that purpose, here, the Paraffin-Embedded-Tissue-blot (PET-blot) method was applied on brains from C57Bl/6 mice infected with cattle BSE, experimental sheep-BSE or Feline Spongiform Encephalopathy (FSE) from a cheetah. The PrPres distribution was comparable whatever the 3 BSE agent sources considered and was distinct from the PrPres distribution in C57Bl/6 mice inoculated with a French scrapie isolate or with a mouse-adapted scrapie strain (C506M3). These data confirm a common origin of infectious agent responsible for the British and French cattle BSE. They also indicate that PET-blot method appears as a precise complementary tool in prion strain studies as it offers an easy and quick assessment of the PrPres mapping. The advantages and limits of the PET-Blot method are discussed and compared with other established and validated methods of strain typing.

Key Words: BSE, mouse, PET-blot, prion, scrapie, strains


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