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JHC exPRESS: First Published May 27, 2005. doi:10.1369/jhc.5B6708.2005
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A more recent version of this article appeared on September 1, 2005.
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Brief Reports

Establishment of Stable GH3 Cell Line Expressing Enhanced Yellow Fluorescein Protein (EYFP)-Growth Hormone (GH) Fusion Protein

Akira Matsuno 1*, Akiko Mizutani 1, Johbu Itoh 1, Susumu Takekoshi 1, Tadashi Nagashima 1, Hiroko Okinaga 1, Koji Takano 1 and R. Yoshiyuki Osamura 1

1 Department of Neurosurgery, Teikyo University Ichihara Hospital, Anesaki, Ichihara City, Chiba, Japan (AMa,TN), Basic Medical Science and Molecular Medicine (AMi), Teaching and Research Support Center (JI), and Department of Pathology (RYO), Tokai University School of Medicine, Boseidai, Isehara City, Kanagawa, Japan, and Division of Endocrinology, Department of Internal Medicine, University of Tokyo Hospital, Hongo, Bunkyo-ku, Tokyo, Japan (HO,KT)

* To whom correspondence should be addressed. E-mail: akirakun{at}med.teikyo-u.ac.jp.

Submitted on April 3, 2005
Accepted on 14 April 2005


  Abstract
To investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of GH linked to EYFP. This stable GH3 cell secretes secretory granules of GH linked to EYFP upon stimulated by Ca2+ influx or Ca2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH.

Key Words: EYFP, GH, GH3 cell


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