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JHC exPRESS: First Published May 27, 2005. doi:10.1369/jhc.5B6713.2005
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on October 1, 2005.
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Brief Reports

Mucin Granules Are in Close Contact with Tubular Elements of the Endoplasmic Reticulum

Juan Perez-Vilar 1*, Carla M. Pedrosa Ribeiro 1, Wendy C. Salmon 1, Raean Mabolo 1 and Richard C. Boucher 1

1 Cystic Fibrosis/Pulmonary Research and Treatment Center, School of Medicine (JP-V,CMPR,RM,RCB) and Michael Hooker Microscopy Facility (WCS), University of North Carolina, Chapel Hill, North Carolina

* To whom correspondence should be addressed. E-mail: juan_vilar{at}med.unc.edu.

Submitted on April 5, 2005
Accepted on 15 April 2005


  Abstract
Live cell imaging methods were used to characterize goblet cells expressing a MUC5AC domain fused to GFP that labels the granule lumen. Golgi complex and endosomes/lysosomes elements largely resided in the periphery of the granular mass. On the contrary, a tubular meshwork of endoplasmic reticulum (ER) was in close contact with the mucin granules. This meshwork could be identified in fixed native human bronchial goblet cells labeled with an anti-calreticulin antibody. The potential biological significance of this ER network for mucin secretion is discussed.

Key Words: goblet cell, mucous cell, mucin granule, endoplasmic reticulum, mucins, organelles, FRAP


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